Enhancing Mass Spectrometry-Based MHC-I Peptide Identification Through a Targeted Database Search Approach.
Enhancing Mass Spectrometry-Based MHC-I Peptide Identification Through a Targeted Database Search Approach.
Methods Mol Biol. 2019;2024:301-307
Authors: Konda P, Murphy JP, Nielsen M, Gujar S
Abstract
MHC-bound peptide ligands dictate the activation and specificity of CD8+ T- cells-based and thus are important for devising T-cell immunotherapies. In recent times, advances in mass spectrometry (MS) have enabled the precise identification of these peptides, wherein MS/MS spectra are compared against a reference proteome. Unfortunately, matching immunopeptide MS/MS to reference proteome databases is hindered by inflated search spaces attributed to the number of matches that need to be considered due to a lack of enzyme restriction. These large search spaces limit the efficiency with which MHC-I peptides are identified. Here we offer a solution to this problem whereby we describe a targeted database search approach and accompanying tool SpectMHC that is based on a priori predicted MHC-I peptides (Murphy et al., J Proteome Res 16:1806-1816, 2017).
PMID: 31364058 [PubMed - in process]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Konda P, Murphy JP, Nielsen M, Gujar S Tags: Methods Mol Biol Source Type: research