Preferential uptake of chitosan-coated PLGA nanoparticles by primary human antigen presenting cells

Publication date: Available online 31 July 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Verónica Durán, Hanzey Yasar, Jennifer Becker, Durairaj Thiyagarajan, Brigitta Loretz, Ulrich Kalinke, Claus-Michael LehrAbstractBiodegradable polymeric nanoparticles (NP) made from poly (lactid-co-glycolide) acid (PLGA) and chitosan (CS) hold promise as innovative formulations for targeted delivery. Since interactions of such NP with primary human immune cells have not been characterized, yet, here we assessed the effect of PLGA or CS-PLGA NP treatment on human peripheral blood mononuclear cells (PBMC), as well as on monocyte-derived DC (moDC). Amongst PBMC, antigen presenting cells (APC) showed higher uptake of both NP preparations than lymphocytes. Furthermore, moDC internalized CS-PLGA NP more efficiently than PLGA NP, presumably because of receptor-mediated endocytosis. Consequently, CS-PLGA NP were delivered mostly to endosomal compartments, whereas PLGA NP primarily ended up in lysosomes. Thus, CS-PLGA NP confer enhanced delivery to endosomal compartments of APC, offering new therapeutic options to either induce or modulate APC function and to inhibit pathogens that preferentially infect APC.Graphical AbstractPLGA and chitosan-coated PLGA nanoparticles (NP) were synthesized and their interaction with human primary immune cells was evaluated. Within PBMC, antigen presenting cells (APC), including monocytes and DC, took up both NP preferentially. Further ...

Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 1, 2019 Category: Nanotechnology Source Type: research