Differential in vivo biodistribution of 131I-labeled exosomes from diverse cellular origins and its implication for theranostic application

Publication date: Available online 1 August 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Mohammad H. Rashid, Thaiz F. Borin, Roxan Ara, Kartik Angara, Jingwen Cai, Bhagelu R. Achyut, Yutao Liu, Ali S. ArbabAbstractExosomes are critical mediators of intercellular crosstalk and are regulator of the cellular/tumor microenvironment. Exosomes have great prospects for clinical application as a theranostic and prognostic probe. Nevertheless, the advancement of exosomes research has been thwarted by our limited knowledge of the most efficient isolation method and their in vivo trafficking. Here we have shown that a combination of two size-based methods using a 0.20 μm syringe filter and 100 k centrifuge membrane filter followed by ultracentrifugation yields a greater number of uniform exosomes. We also demonstrated the visual representation and quantification of the differential in vivo distribution of radioisotope 131I-labeled exosomes from diverse cellular origins, e.g., tumor cells with or without treatments, myeloid-derived suppressor cells and endothelial progenitor cells. We also determined that the distribution was dependent on the exosomal protein/cytokine contents. The applied in vivo imaging modalities can be utilized to monitor disease progression, metastasis, and exosome-based targeted therapy.Graphical AbstractExosomes are biological nano-particles, which have immense prospects for clinical application as a diagnostic marker, monitoring ...

Source: Nanomedicine: Nanotechnology, Biology and Medicine - August 1, 2019 Category: Nanotechnology Source Type: research