Effect of bovine genotype on innate immune response of heifers to repeated lipopolysaccharide (LPS) administration

Publication date: Available online 31 July 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Georgina Cousillas Boam, Wanda J. Weber, Aimee Benjamin, Stanislaw Kahl, G. Allen Bridges, Theodore H. Elsasser, David Kerr, Brian A. CrookerAbstractThis pilot study provides a preliminary assessment of the impact of genotype on acute innate immune pro-inflammatory, metabolic and endocrine responses to repeated lipopolysaccharide (LPS) administered to growing heifers. Heifers (n = 4/genotype) were from unselected (stable milk yield since 1964, UH) or contemporary (CH) Holstein cows that differed in milk yield (6,200 vs 11,100 kg milk/305 d) or from contemporary Black Angus (CA) cows bred to contemporary Red Angus bulls. Heifers were challenged with iv administration of 0.5 μg LPS/kg body weight on day 1 (Challenge 1) and d 5 (Challenge 2) of study to assess endotoxin tolerance. Plasma was collected at -1, -0.5, 0, 1, 2, 3, 4, 6, 8, and 24 h relative to each LPS administration. Rectal body temperature (BT) was measured before each blood sampling and at 5 and 7 h. Data were analyzed by repeated measures with sampling time as the repeated effect. Each genotype had at least one pro-inflammatory response that indicated it might have a more robust response than the other genotypes. The CH heifers had a greater TNF-α response, UH heifers had greater IL-6 and XO responses and CA heifers had greater BT and SAA response to LPS than the other genotypes. There was a ge...
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research