Slow growth determines nonheritable antibiotic resistance in Salmonella enterica

Bacteria can withstand killing by bactericidal antibiotics through phenotypic changes mediated by their preexisting genetic repertoire. These changes can be exhibited transiently by a large fraction of the bacterial population, giving rise to tolerance, or displayed by a small subpopulation, giving rise to persistence. Apart from undermining the use of antibiotics, tolerant and persistent bacteria foster the emergence of antibiotic-resistant mutants. Persister formation has been attributed to alterations in the abundance of particular proteins, metabolites, and signaling molecules, including toxin-antitoxin modules, adenosine triphosphate, and guanosine (penta) tetraphosphate, respectively. Here, we report that persistent bacteria form as a result of slow growth alone, despite opposite changes in the abundance of such proteins, metabolites, and signaling molecules. Our findings argue that transitory disturbances to core activities, which are often linked to cell growth, promote a persister state regardless of the underlying physiological process responsible for the change in growth.
Source: Signal Transduction Knowledge Environment - Category: Science Authors: Tags: STKE Research Articles Source Type: news

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(University of Gothenburg) A hitherto unknown antibiotic-resistant bacteria species, in the same family as E. coli and Salmonella spp., has been found and classified in Sweden. The proposed taxonomic name of the species -- the first of the new genus -- is Scandinavium goeteborgense, after the city of Gothenburg, Sweden, where the bacterium was isolated and the research was done.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news
Publication date: 1 March 2020Source: Materials Chemistry and Physics, Volume 243Author(s): Samir Bouchareb, Rachida Doufnoune, Farid Riahi, Hafsa Cherif-Silini, Lassaad BelbahriAbstractGO-Ag nanohybrids have attracted tremendous attention thanks to their several potential properties. In this research work, a new approach was adopted, where Triphenylphosphine (PPh3) was used as a linkage to decorate the surface of Graphene oxide (GO) nanosheets by Silver nanoparticles (AgNPs) via a simple method. The Fourier transform infrared spectroscopy (FTIR), Ultraviolet–visible spectroscopy (UV–Vis), X-ray diffraction ana...
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research
In this study, bacteriophage LPST144 against S. Typhimurium (ATCC13311) was isolated from sewage and selected owing to its excellent lytic capacity. After genome sequence analysis, the endolysin from LPST144 was cloned and expressed, and its lytic activity was verified. Morphological analysis showed that bacteriophage LPST144 belongs to Podoviridae family and Caudovirales order, and could completely inhibit host bacterial growth within 7 h at multiplicity of infection of 0.01–1000. Genome analysis indicated LPST144 genome comprised a 39,050 bp DNA with 43 putative open reading frames, of which 27 were annotated t...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
In this study, we examined the genome sequences of 2239 bacteriophages from different sources for the presence of ARGs. The identified ARG-carrying bacteriophages were then analyzed by PHACTS, PHAST, and HostPhinder programs to determine their lifestyles, genes coding for bacterial cell lysis, recombinases, and a spectrum of their potential host species, respectively. We employed the SplitsTree, RDP4 and SimPlot software packages in recombination tests to identify HGT events of ARGs between these bacteriophages and bacteria. In our analyses, some ARG-carrying bacteriophages exhibited temperate and/or polyvalent patterns. T...
Source: Current Microbiology - Category: Microbiology Authors: Tags: Curr Microbiol Source Type: research
The rapidly increasing prevalence and spread of antibiotic-resistant Salmonella worldwide have become a thorny problem that poses a serious threat to human health. It is speculated that antibiotic abuse, frequent traveling, and mass gatherings accelerate this threat. To explore this hypothesis, we investigated 13 Salmonella isolates from Medina, Saudi Arabia and 15 from China as the control group using typical methods of serotype identification, antibiotic resistance tests, pulsed-field gel electrophoresis (PFGE), and multi-locus sequence typing (MLST). Our results indicated that the isolates from China showed greater sero...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
In conclusion, to best of our knowledge these findings are the first report of isolation of 4-bromophenol and Bis (2-ethylhexyl) phthalate from genus Nocardiopsis, thus suggesting that rare actinomycetes are promising source of therapeutically important bioactive metabolites.
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
THURSDAY, Oct. 3, 2019 -- In a finding that suggests organic is best, a new study indicates that chickens raised without antibiotics may have fewer types of antibiotic-resistant salmonella than animals raised at factory farms. Salmonella is a...
Source: Drugs.com - Daily MedNews - Category: General Medicine Source Type: news
Basamba Chicken litter application on land as an organic fertilizer is the cheapest and most environmentally safe method of disposing of the volume generated from the rapidly expanding poultry industry worldwide. However, little is known about the safety of chicken litter for land application and general release into the environment. Bridging this knowledge gap is crucial for maximizing the benefits of chicken litter as an organic fertilizer and mitigating negative impacts on human and environmental health. The key safety concerns of chicken litter are its contamination with pathogens, including bacteria, fungi, hel...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Review Source Type: research
Nature, Published online: 04 September 2019; doi:10.1038/s41586-019-1521-8The re-seeding of antibiotic-resistant persister subpopulations of Salmonella enterica into the gut lumen favours the transfer of resistance plasmids to gut-resident enterobacteria, showing that even small reservoirs of persister bacteria facilitate the spread of antibiotic resistance.
Source: Nature AOP - Category: Research Authors: Source Type: research
Natural microcin J25 (MccJ25) represent promising alternatives to traditional antibiotics for the treatment of drug-resistant infections. However, little is known about the antibacterial activity of recombinant MccJ25 against foodborne pathogens. Here, the activity of recombinant MccJ25 was examined using a matrix of conditions in order to assess the efficacy of recombinant MccJ25 as a mitigation against foodborne pathogens, Salmonella species and Escherichia coli (E. coli) O157. Results showed that recombinant MccJ25 displayed excellent antimicrobial activity against these foodborne pathogens, including clinical isolates ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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