High-throughput screening for negative-stranded hemorrhagic fever viruses using reverse genetics.

High-throughput screening for negative-stranded hemorrhagic fever viruses using reverse genetics. Antiviral Res. 2019 Jul 26;:104569 Authors: Wendt L, Bostedt L, Hoenen T, Groseth A Abstract Viral hemorrhagic fevers (VHFs) cause thousands of fatalities every year, but the treatment options for their management remain very limited. In particular, the development of therapeutic interventions is restricted by the lack of commercial viability of drugs targeting individual VHF agents. This makes approaches like drug repurposing and/or the identification of broad range therapies (i.e. those directed at host responses or common proviral factors) highly attractive. However, the identification of candidates for such antiviral repurposing or of host factors/pathways important for the virus life cycle is reliant on high-throughput screening (HTS). Recently, such screening work has been increasingly facilitated by the availability of reverse genetics-based approaches, including tools such as full-length clone (FLC) systems to generate reporter-expressing viruses or various life cycle modelling (LCM) systems, many of which have been developed and/or greatly improved during the last years. In particular, since LCM systems are capable of modelling specific steps in the life cycle, they are a valuable tool for both targeted screening (i.e. for inhibitors of a specific pathway) and mechanism of action studies. This review seeks to summarize the curre...
Source: Antiviral Research - Category: Virology Authors: Tags: Antiviral Res Source Type: research