An arsenal of methods for the experimental characterization of intrinsically disordered proteins - How to choose and combine them?

An arsenal of methods for the experimental characterization of intrinsically disordered proteins - How to choose and combine them?
Arch Biochem Biophys. 2019 Jul 26;:
Authors: Schramm A, Bignon C, Brocca S, Grandori R, Santambrogio C, Longhi S
Abstract
In this review, we detail the most common experimental approaches to assess and characterize protein intrinsic structural disorder, with the notable exception of NMR and EPR spectroscopy, two ideally suited approaches that will be described in depth in two other reviews within this special issue. We discuss the advantages, the limitations, as well as the caveats of the various methods. We also describe less common and more demanding approaches that enable achieving further insights into the conformational properties of IDPs. Finally, we present recent developments that have enabled assessment of structural disorder in living cells, and discuss the currently available methods to model IDPs as conformational ensembles.
PMID: 31356778 [PubMed - as supplied by publisher]

Source: Archives of Biochemistry and Biophysics - July 25, 2019 Category: Biochemistry Authors: Schramm A, Bignon C, Brocca S, Grandori R, Santambrogio C, Longhi S Tags: Arch Biochem Biophys Source Type: research