Quantitative secretome analysis unravels new secreted proteins in Amphotericin B resistant Leishmania donovani.

Quantitative secretome analysis unravels new secreted proteins in Amphotericin B resistant Leishmania donovani. J Proteomics. 2019 Jul 26;:103464 Authors: Garg G, Ali V, Singh K, Gupta P, Ganguly A, Sahasrabuddhe AA, Das P Abstract Leishmaniasis is second most neglected disease after malaria and seems to be a worldwide concern because of increased drug resistance and non-availability of approved vaccine. The underlying molecular mechanism of drug resistance (Amp B) in Leishmania parasites still remains elusive. Herein, the present study investigated differentially expressed secreted proteins of Amphotericin B sensitive (S) and resistant (R) isolate of Leishmania donovani by using label free quantitative LC-MS/MS approach. A total of 406 differentially expressed secreted proteins were found between sensitive (S) and resistant (R) isolate. Among 406 proteins, 32 were significantly up regulated (>2.0 fold) while 22 were down regulated (<0.5 fold) in resistant isolate of L. donovani. Further, differentially expressed proteins were classified into 11 various biological processes. Interestingly, identified up regulated proteins in resistant parasites were dominated in carbohydrate metabolism, stress response, transporters and proteolysis. Western blot and enzymatic activity of identified proteins validate our proteomic findings. Finally, our study demonstrated some new secreted proteins associated with Amp B resistance which provides...
Source: Journal of Proteomics - Category: Biochemistry Authors: Tags: J Proteomics Source Type: research