Quantification of microcystins in natural waters by HPLC-UV after a pre-concentration step: validation of the analytical performances and study of the interferences

Publication date: Available online 29 July 2019Source: Environmental Toxicology and PharmacologyAuthor(s): Hélène Thuret-Benoist, Virginie Pallier, Geneviève Feuillade-CathalifaudAbstractA method for the determination of microcystins concentrations (MC-LR, MC-RR, MC-YR) in natural water samples was optimized using High Performance Liquid Chromatography with UV/PDA detection after Solid Phase Extraction. Solid Phase Extraction is needed to clean natural sample and concentrate pollutant. The method was validated by evaluation of specificity and repeatability. Average recoveries in ultra-pure grade water were better than 95% with Relative Standard Deviation values lower than 4%. Matrix interferences, as pH, conductivity and organic matter content, were tested. pH must be fixed between 6 and 8 to avoid under-estimation or over-estimation and conductivity did not interfere with the analytical method. Organic Matter content negatively impacted microcystins quantification unlike organic matter characteristics. It over-estimated the concenration by an average of 19%. Then, the developed method was applied to study the occurrence of microcystins in Pigeard pond (France). These results constitute the first report on the concentration levels and seasonal variations of microcystins in this resource water.Graphical abstract
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research

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CONCLUSION: In the outpatient clinic specializing in oral anticoagulation, patients add more specific beliefs about the interference of vitamin K-rich diet on coagulation, when compared to those in the general cardiology outpatient clinic. PMID: 31618372 [PubMed - in process]
Source: Revista gaucha de enfermagem EENFUFRGS - Category: Nursing Tags: Rev Gaucha Enferm Source Type: research
CONCLUSION: The Tracer Methodology is used for evaluating and improving the quality of care. PMID: 31618371 [PubMed - in process]
Source: Revista gaucha de enfermagem EENFUFRGS - Category: Nursing Tags: Rev Gaucha Enferm Source Type: research
Publication date: Available online 16 October 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Nicole E. Breuner, Shelby L. Ford, Andrias Hojgaard, Lynn M. Osikowicz, Christina M. Parise, Maria F. Rosales Rizzo, Ying Bai, Michael L. Levin, Rebecca J. Eisen, Lars EisenAbstractThe invasive, human-biting Asian longhorned tick, Haemaphysalis longicornis, was detected in New Jersey in the eastern United States in August of 2017 and by November of 2018 this tick had been recorded from 45 counties across 9 states, primarily along the Eastern Seaboard. The establishment of H. longicornis in the United States has raised the ques...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: Available online 16 October 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Jinming Wang, Jifei Yang, Shandian Gao, Aihong Liu, Muhammad Rashid, Youquan Li, Zhijie Liu, Junlong Liu, Guangyuan Liu, Jianxun Luo, Guiquan Guan, Hong YinAbstractBovine theileriosis, caused by protozoan parasites of the genus Theileria, presents with various clinical symptoms. In cattle, clinical presentations and outcomes of bovine theileriosis are closely correlated with the causative Theileria spp. Thus, accurate detection and discrimination of Theileria spp. are essential for epidemiological studies and for provision of ...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: Available online 16 October 2019Source: Small Ruminant ResearchAuthor(s): Alison M. Lee, Alan Wolfe, Joseph P. Cassidy, John Moriarty, Ronan O’Neill, Claire Fahy, Emily Connaghan, Chris Cousens, Mark P. Dagleish, Maire C. McElroy, Locksley L. McV. MessamAbstractJaagsiekte sheep retrovirus (JSRV) is the causative agent of ovine pulmonary adenocarcinoma (OPA). JSRV infection is usually detected post-mortem by macroscopic and histological examination of lungs for lesions of OPA. Subsequently, the presence of JSRV may be confirmed using polymerase chain reaction (PCR) on tumour tissue. Our goal was to d...
Source: Small Ruminant Research - Category: Zoology Source Type: research
This study not only represents the most systematic analysis of codon usage patterns in PEDV orf3 genes, but also provides a basic shaping mechanism of the codon usage bias.
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Publication date: Available online 16 October 2019Source: Research in Veterinary ScienceAuthor(s): Baolin Song, Qianmei Zeng, Yuan Liu, Bangyuan WuAbstractAbundant evidence now supports the idea that methionine deficiency has negative effects on chicken healthy especially in the aspect of cell cycle regulation and apoptosis. But, lacking of knowledge is the evaluation on metabolic organs. To test the effect of methionine deficiency on the kidney, we assessed the apoptosis and the cell cycle of kidney induced by methionine deficiency by the methods of TdT-mediated dUTP Nick-End Labeling (TUNEL), flow cytometry (FCM), quanti...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Publication date: Available online 16 October 2019Source: Research in Veterinary ScienceAuthor(s): Pascal Rainard
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
Publication date: Available online 16 October 2019Source: Research in Veterinary ScienceAuthor(s): L.C. Rhades, M. Larzábal, A. Bentancor, J. Sabio y García, F.J. Babinec, A. Cataldi, N. Amigo, V.N. Baldone, L. Urquiza, P.J. Delicia, M.C. FortAbstractBovines are the primary reservoir of enterohemorrhagic Escherichia coli (EHEC) O157:H7 and the main source of its transmission to humans. Here, we present a one-year longitudinal study of fecal shedding of E. coli O157. E. coli O157 obtained from recto-anal mucosal samples were characterized by multiplex PCR. The E. coli O157 prevalence ranged from 0.84% in July ...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
PMID: 31617895 [PubMed - in process]
Source: Endocrinology - Category: Endocrinology Authors: Tags: Endocrinology Source Type: research
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