Synergistic effect of 2D material coated Pt nanoparticles with PEDOT polymer on electrode surface interface for a sensitive label free Helicobacter pylori CagA(Ag-Ab) immunosensing.

Synergistic effect of 2D material coated Pt nanoparticles with PEDOT polymer on electrode surface interface for a sensitive label free Helicobacter pylori CagA(Ag-Ab) immunosensing. Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109733 Authors: Gupta S, Tiwari A, Jain U, Chauhan N Abstract Helicobacter pylori (H. pylori) immunosensor based on platinum nanoparticles/poly(3,4-ethylenedioxythiophene)/reduced graphene oxide (Ptnano/PEDOT/red-GOx) modified gold electrode (Au-ET) was stepwise fabricated for the detection of cytotoxin-associated gene A antibody (CagA antibody). H. pylori is a microaerophillic and a Gram-negative bacteria that causes gastric ulcer leading eventually to adenocarcinoma (gastric cancer) in the later stage. H. pylori colonizes inner lining of human stomach. The developed diagnostic sensing interface would allow H. pylori (stomach infection) detection in early stage and would be a great contribution in clinical laboratories. In order to fabricate the immunosensor, CagA antigen was immobilized over the Ptnano/PEDOT/red-GOx modified Au-ET. Afterwards, the modified electrode was used for immuno-sensing of H. pylori specific Cag A antibodies in serum. At lower voltage the modified Ptnano/PEDOT/red-GOx/Au-ET shows an amplified sensing at the interface that makes the sensor more sensitive and specific. CagA is a virulence factor produced by H. pylori was determined by sudden decrease in the current. The laboratory synthesized nano composite...
Source: Appl Human Sci - Category: Physiology Authors: Tags: Mater Sci Eng C Mater Biol Appl Source Type: research

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Authors: Herth FJF, Reinmuth N, Wormanns D, Antoch G, Biederer J, Vogel-Claussen J, Kauczor HU, Pfeifer M, Vorstand der Deutschen Gesellschaft für Pneumologie und Beatmungsmedizin, Vorstand der Deutschen Röntgengesellschaft Abstract Substantial new data on early detection of lung cancer with low-dose CT has become available since the last joint statement of the German Roentgenological Society and the German Respiratory Society was published in 2011. The German S3 guideline on lung cancer was revised in 2018 and now contains a weak recommendation towards early detection of lung cancer with low-dose CT in a...
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Publication date: 21–27 September 2019Source: The Lancet, Volume 394, Issue 10203Author(s): The Lancet
Source: The Lancet - Category: General Medicine Source Type: research
Publication date: Available online 9 May 2019Source: Materials Science and Engineering: CAuthor(s): Shaivya Gupta, Ashutosh Tiwari, Utkarsh Jain, Nidhi ChauhanAbstractHelicobacter pylori (H. pylori) immunosensor based on platinum nanoparticles/poly(3,4-ethylenedioxythiophene)/reduced graphene oxide (Ptnano/PEDOT/red-GOx) modified gold electrode (Au-ET) was stepwise fabricated for the detection of cytotoxin-associated gene A antibodies (CagA antibody). H. pylori is a microaerophillic and a Gram-negative bacteria that causes gastric ulcer leading eventually to adenocarcinoma (gastric cancer) in the later stage. H. pylori col...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Abstract Helicobacter pylori (H. pylori) is an organism that is widespread in the human population and is sometimes responsible for some of the most common chronic clinical disorders of the upper gastrointestinal tract in humans, such as chronic-active gastritis, duodenal and gastric ulcer disease, low-grade B-cell mucosa associated lymphoid tissue lymphoma of the stomach, and gastric adenocarcinoma, which is the third leading cause of cancer death worldwide. The routes of infection have not yet been firmly established, and different routes of transmission have been suggested, although the most commonly accepted h...
Source: World Journal of Gastroenterology : WJG - Category: Gastroenterology Authors: Tags: World J Gastroenterol Source Type: research
Abstract AimsMost gastric carcinomas develop in association with mucosal atrophy and hypochlorhydria, whereas benign peptic ulcers are acidÔÇÉrelated. Given that acid sterilizes gastric contents, we hypothesized that ulcerated gastric cancers may be associated with increased luminal microbes compared with peptic ulcers, and that this feature may represent a helpful diagnostic clue to the presence of malignancy. We performed this study to determine whether features of luminal debris, including microorganisms, from ulcerated gastric cancers were significantly different from those of debris associated with benign ulcers. Meth...
Source: Histopathology - Category: Pathology Authors: Tags: Original Article Source Type: research
Date: July 17, 2017 Issue #:  1525Summary:  About 50% of the world ’s population is infected withHelicobacter pylori. These gastric bacteria can cause chronic inflammation and have been associated with development of gastritis, peptic ulcer disease, gastric adenocarcinoma, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma. Eradication ofH. pylori can promote gastric healing, prevent recurrence of duodenal and gastric ulcers, and reduce the incidence of gastric cancer. Guidelines for treatment ofH. pylori infection were updated recently.
Source: The Medical Letter - Category: Drugs & Pharmacology Authors: Tags: Alinia Amoxicillin Amoxil Antibacterials Biaxin Bismuth subsalicylate BreathID BreathTek Clarithromycin Clostridium difficile Clostridium difficile associated disease Diazepam Doxycycline Esomeprazole Flagyl Fluoroquinolones Source Type: research
Abstract Helicobacter pylori (H. pylori) is a gastric human pathogen associated with acute and chronic gastritis, 70% of all gastric ulcers, 85% of all duodenal ulcers, and both forms of stomach cancer, mucosal-associated lymphoid tissue (MALT) lymphoma and adenocarcinoma. Recently, attention has focused on possible relationship between presence of certain virulence factor and H. pylori-associated diseases. Some contradictory data between this bacterium and related disorders has been observed since not all the colonized individuals develop to severe disease. The reported diseases plausibility related to H. pylori ...
Source: World Journal of Gastroenterology : WJG - Category: Gastroenterology Authors: Tags: World J Gastroenterol Source Type: research
Conclusions:H. pylori infection in southeastern region of Turkey with are comparable to those in developed countries.Patients withcagA- andvacA-positiveH. pylorihavea higher risk of severe inflammation and atrophy and should therefore be monitored for the development of gastric cancer.
Source: The Journal of Infection in Developing Countries - Category: Infectious Diseases Source Type: research
This study aimed to evaluate the effect of the expression level of NOD1 and NOD2 on the susceptibility to gastric cancer as well as peptic ulcer in individuals with H. pylori infection. The gene expression levels of these molecules were compared in three groups of non-ulcer dyspepsia (NUD) as a control group (n=52); peptic ulcer disease (PUD), (n=53); and gastric cancer (GC), (n=39). Relative expression levels of NOD1 in patients with GC were higher than those of NUD and PUD (p
Source: Iranian Journal of Allergy, Asthma and Immunology - Category: Allergy & Immunology Authors: Tags: Iran J Allergy Asthma Immunol Source Type: research
In conclusion, the oral cavity is an extra-gastric reservoir of HP and periodontal therapy associated with systemic therapy can better eradicate HP from the mucosa of all gastro-enteric tract, reducing relapse of HP infection. Prospective cohort studies are needed to demonstrate the bacterial action in the oral cavity. PMID: 26511191 [PubMed - in process]
Source: Journal of Biological Regulators and Homeostatic Agents - Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research
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