Protective effects of delphinidin against H2O2 ‑induced oxidative injuries in human retinal pigment epithelial cells.

Protective effects of delphinidin against H2O2‑induced oxidative injuries in human retinal pigment epithelial cells. Biosci Rep. 2019 Jul 25;: Authors: Ni T, Yang W, Xing Y Abstract Age-related macular degeneration (AMD) is now one of the leading causes of blindness in the elderly population and oxidative stress-induced damage to retinal pigment epithelial (RPE) cells occurs as part of the pathogenesis of AMD. In the present study, we evaluated the protective effect of delphinidin against H2O2-induced toxicity in human ARPE-19 cells and its molecular mechanism. MTT assay and flow cytometry demonstrated that pretreatment of ARPE-19 cells with delphinidin (25, 50 and 100μg/ml) significantly increased cell viability and reduced the apoptosis from H2O2 (0.5 mM)-induced oxidative stress in a concentration-dependent manner, which was achieved by the inhibition of Bax, cytochrome c, and caspase-3 protein expression and enhancement of Bcl-2 protein. The same tendency was observed in ARPE-19 cells pre-treated with 15 mM of N-acetylcysteine (NAC) before the addition of H2O2 Furthermore pre-incubation of ARPE-19 cells with delphinidin markedly inhibited the intracellular ROS generation and Nox1 protein expression induced by H2O2 Moreover, the decreased antioxidant enzymes activities of SOD, CAT, and GSH-PX and elevated MDA level in H2O2-treated cells were reversed to the normal standard by the addition of delphinidin, which was regulated by ...
Source: Bioscience Reports - Category: Biomedical Science Authors: Tags: Biosci Rep Source Type: research