Freeze ‐dry processing of three‐dimensional cell constructs for bone graft materials

In this study, we found that sucrose‐containing phosphate buffer was suitable for freeze drying to maintain minerals and protein functions within 3D cell constructs, w hereas citrate buffer was inappropriate. The insights gained by this study may facilitate the development of novel cell‐based biomaterials fabricated by tissue engineering approaches and bone graft biomaterials.

Source: Journal of Biomedical Materials Research Part B: Applied Biomaterials - July 25, 2019 Category: Materials Science Authors: Jun ‐Ichi Sasaki, Itsumi Yoshimoto, Chihiro Katata, Ririko Tsuboi, Satoshi Imazato Tags: ORIGINAL RESEARCH REPORT Source Type: research