Comparison of two recombinant systems for expression of cholera toxin B subunit from Vibrio cholerae

Conclusion: Expression of rCTB in pAE_ctxB construct was more efficient (15-fold) than pQE_ctxB, and it seems that Lac UV5 in E. coli BL21 (DE3) is more compatible with the former construct. This expression system can be used to produce recombinant CTB in high yield which may enable us to study the oral tolerance or mucosal adjuvant properties of rCTB using animal models.
Source: Indian Journal of Medical Microbiology - Category: Microbiology Authors: Source Type: research