How do you calculate a brain tumor's MIB-1 index?

Elizabeth J. Cochran, MDI was recently contacted by the affable Elizabeth Cochran, MD  asking if I would post a query regarding MIB-1 counts on brain tumors. Does anyone know if there is a standardized approach to this?  Please tell us your approach to MIB-1 quantification in the comment section. Dr. Cochran takes an approach based on an article she had read in Human Pathology some time ago, summarized as follows: 1. Examine control section stained with MIB-1 antibody to confirm that the stain worked.2. Survey the slide of the case to be counted to find the area(s) that have the most stained nuclei.3. Put reticule in right eyepiece of microscope.4. Place the area identified in #2 under the reticule at 40x power.5. Using the hand held counter, count all the immunoreactive nuclei within the entire grid.6. Count all nuclei that touch the right and bottom outer border of the grid, but NOT the nuclei that touch the upper and left borders.7. Record this number in the lab book.8. Count all nuclei present within one vertical column of the grid.  This column should be randomly chosen.9. Record this number in the lab book.10. Count all nuclei present within one horizontal column of the grid.  Again this column should be randomly chosen, and recorded in the lab book.11. Again, be sure to count all nuclei that touch the right and bottom outer border of the grid, but NOT the nuclei that touch the upper and left borders12. Repeat this procedure for at least two more hig...
Source: neuropathology blog - Category: Pathologists Tags: neoplasms neuropathologists Source Type: blogs