The use of chicken and insect infection models to assess the virulence of African Salmonella Typhimurium ST313

by Lizeth Lacharme-Lora, Si ân V. Owen, Richard Blundell, Rocío Canals, Nicolas Wenner, Blanca Perez-Sepulveda, Wai Yee Fong, Rachel Gilroy, Paul Wigley, Jay C. D. Hinton Over recent decades,Salmonella infection research has predominantly relied on murine infection models. However, in many cases the infection phenotypes ofSalmonella pathovars in mice do not recapitulate human disease. For example,Salmonella Typhimurium ST313 is associated with enhanced invasive infection of immunocompromised people in Africa, but infection of mice and other animal models with ST313 have not consistently reproduced this invasive phenotype. The introduction of alternative infection models could help to improve the quality and reproducibility of pathogenesis research by facilitating larger-scale experiments. To investigate the virulence ofS. Typhimurium ST313 in comparison with ST19, a combination of avian and insect disease models were used. We performed experimental infections in five lines of inbred and one line of outbred chickens, as well as in the alternative chick embryo andGalleria mellonella wax moth larvae models. This extensive set of experiments identified broadly similar patterns of disease caused by the African and global pathovariants ofSalmonella Typhimurium in the chicken, the chicken embryo and insect models. A comprehensive analysis of all the chicken infection experiments revealed that the African ST313 isolate D23580 had a subtle phenotype of reduced levels of ...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research

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This study quantified the association of rodent fruit damage and the microbiological quality of irrigation water on the risk of microbiological contamination of strawberries collected from 18 U-pick farms across five different districts in the Guangzhou metropolitan region of southern China. Fifty-four composite strawberries samples, with or without evidence of rodent or avian foraging damage (i.e., bitten), along with 16 irrigation water samples, were collected during the spring of 2014 and winter of 2015 from our cohort of 18 farms. Composite strawberry samples and irrigation water were analyzed for total coliforms, E. c...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
Conclusions: The inhalation challenge test using sterilized avian egg may be safe and useful for conforming the diagnosis of bird-related hypersensitivity pneumonia.
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: ILD/DPLD of known origin Source Type: research
Most Salmonella serovars cause disease in many host species, while a few serovars have evolved to be host specific. Very little is known about the mechanisms that contribute to Salmonella host specificity. We compared the interactions between chicken primary macrophages (CDPM) and host-generalist serovar Salmonella enterica serovar Typhimurium, host-adapted Salmonella enterica serovar Dublin, and avian host-specific Salmonella enterica serovar Gallinarum. S. Gallinarum was taken up in lower numbers by CDPM than S. Typhimurium and S. Dublin; however, a higher survival rate was observed for this serovar. In addition, S. Typh...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Bacterial Infections Source Type: research
Publication date: Available online 12 November 2019Source: Veterinary MicrobiologyAuthor(s): Sahar Rostami, Jalil Mehrzad, Ramak Yahyaraeyat, Taghi Zahraei SalehiAbstractSalmonella enterica Subsp enterica serovar Typhimurium (S. Typhimurium, ST) is one of the most important serovars of the genus Salmonella in human and animals. Because of its intracellular tropism, monocytes/macrophages are pivotal in killing of Salmonella serovars; they are also responsible for transporting of ST to extra-intestinal organs. To investigate the effect of the ST on the functions of avian innate immune cells, almost homogeneous enriched monoc...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Publication date: Available online 4 November 2019Source: Preventive Veterinary MedicineAuthor(s): Carla Correia-Gomes, Nick SparksAbstractBackyard poultry producers have been associated with outbreaks of exotic (e.g. avian influenza) and endemic (e.g. Salmonella) disease all over the world. Currently in the UK the registration of small flocks (less than 50 birds) with local authorities is voluntary therefore there is not an accurate record of how many keepers and birds there are or where they are located. This lack of information (e.g. how many birds they keep, what type of birds, biosecurity measures they implement, etc....
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
IJERPH, Vol. 16, Pages 4235: Salmonella in Retail Food and Wild Birds in Singapore—Prevalence, Antimicrobial Resistance, and Sequence Types International Journal of Environmental Research and Public Health doi: 10.3390/ijerph16214235 Authors: Aung Chen Chau Yap Lim Humaidi Chua Yeo Yap Oh Manogaran Hapuarachchi Maiwald Tee Barkham Koh Gutiérrez Schlundt Ng Non-typhoidal salmonellosis is a leading cause of foodborne zoonosis. To better understand the epidemiology of human salmonellosis, this study aimed to determine the prevalence, antimicrobial res...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
The follicle-associated epithelium (FAE) is a specialized structure that samples luminal antigens and transports them into mucosa-associated lymphoid tissues (MALT). In mammals, transcytosis of antigens across the gut epithelium is performed by a subset of FAE cells known as M cells. Here we show that colony-stimulating factor 1 receptor (CSF1R) is expressed by a subset of cells in the avian bursa of Fabricius FAE. Expression was initially detected using a CSF1R-reporter transgene that also label subsets of bursal macrophages. Immunohistochemical detection using a specific monoclonal antibody confirmed abundant expression ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Publication date: Available online 10 October 2019Source: Comparative Immunology, Microbiology and Infectious DiseasesAuthor(s): Marc. K. Kouam, Herman M.F Biekop, Bridget Katte, Alexis TeguiaAbstractSalmonella infections in poultry farms are overlooked in many African countries; yet these infections are mostly zoonotic with impact on both poultry industry and public health. Considering the impact of Salmonella in laying hens, and the role of laying hens as a source of Salmonella outbreak in human, knowledge of the status of Salmonella on laying hen farms as well as the factors influencing the presence of Salmonella is imp...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
Abstract Salmonella infections in poultry farms are overlooked in many African countries; yet these infections are mostly zoonotic with impact on both poultry industry and public health. Considering the impact of Salmonella in laying hens, and the role of laying hens as a source of Salmonella outbreak in human, knowledge of the status of Salmonella on laying hen farms as well as the factors influencing the presence of Salmonella is important. In a cross sectional study, cloacal swabs were collected from 270 commercial laying hens on 27 farms located in Menoua Division. These samples were cultured on standard media...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research
Publication date: Available online 18 September 2019Source: Preventive Veterinary MedicineAuthor(s): Marisa Peyre, Linda Hoinville, James Njoroge, Angus Cameron, Daniel Traon, Flavie Goutard, Clémentine Calba, Vladimir Grosbois, Alexis Delabouglise, Viktor Varant, Julian Drewe, Dirk Pfeiffer, Barbara HäslerAbstractInformation about infectious diseases at the global level relies on effective, efficient and sustainable national and international surveillance systems. Surveillance systems need to be regularly evaluated to ensure their effectiveness, the quality of the data and information provided, as well as to b...
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
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