Sodium caprylate induced precipitation post protein a chromatography as an effective means for host cell protein clearance

In this study, we replaced CA with its sodium salt – sodium caprylate (SC). For the five mAbs studied, SC has been shown to be equally effective as CA at precipitating HCPs. As the salt form has a higher solubility, SC stock solution with relatively high concentration can be easily prepared, which facilitates its adding to the Protein A elution pool. Thus, this study not only confirms the effectiveness of CA/SC-induced HCP precipitation but also provides a more convenient way to integrate this method into the downstream process.
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research