Assessment of the Effective Impact of Bisphenols on Mitochondrial Activity and Steroidogenesis in a Dose-Dependency in Mice TM3 Leydig Cells.

Assessment of the Effective Impact of Bisphenols on Mitochondrial Activity and Steroidogenesis in a Dose-Dependency in Mice TM3 Leydig Cells.
Physiol Res. 2019 Jul 25;
Authors: Jambor T, Kovacikova E, Greifova H, Kovacik A, Libova L, Lukac N
Abstract
reproductive disorders in both sexes. The aim of the present in vitro study was to evaluate the potential impact bisphenols A, B, S and F on the cell viability and testosterone release in TM3 Leydig cell line. Mice Leydig cells were cultured in the presence of different concentrations of bisphenols (0.04-50 µg.ml-1) during 24 h exposure. Quantification of the cell viability was assessed using the metabolic activity assay, while the level of testosterone in cell culture media was determined by enzyme-linked immunosorbent assay. Within the panel of substances under investigations, the higher experimental concentrations (10, 25 and 50 µg.ml-1) significantly (P<0.001) decreased Leydig cells viability, while the same doses of BPA and BPB also reduced testosterone production significantly (P<0.001). Taken together, the results of our study reported herein is a consistent with the conclusion that higher experimental doses of bisphenols have a cytotoxic effect and could have a dose-dependent impact on testosterone production.
PMID: 31342755 [PubMed - as supplied by publisher]

Source: Physiological Research - July 24, 2019 Category: Physiology Authors: Jambor T, Kovacikova E, Greifova H, Kovacik A, Libova L, Lukac N Tags: Physiol Res Source Type: research