Mimicking the cauda epididymal plasma-like osmolality in extender improves liquid preservation of ram semen at 3-5 °C.

In conclusion, semen extender having CEP-like osmolality but not the pH was superior to extenders having conventional osmolality and pH for liquid preservation of ram semen. Abbreviations: AI: artificial insemination; ALH: amplitude of lateral head displacement; BCF: beat cross frequency; CASA: computer-assisted semen analyzer; CEP: cauda epididymal plasma; ELON: elongation; EYTF: egg yolk-Tris-citrate-fructose; FMI: functional membrane integrity; GLM: general linear model; GPC: glycerophosphatidylcholine; HOS: hypoosmotic swelling; LIN: linearity; pHe: external pH; PROG: progressive motility; S.E.M.: standard error of the mean; SLTF: soya lecithin-Tris-fructose extender; SP: seminal plasma; STR: straightness; VAP: average path velocity; VCL: curvilinear velocity; VSL: straight-line velocity; TM: total motility. PMID: 31339369 [PubMed - as supplied by publisher]

https://www.ncbi.nlm.nih.gov/pubmed/31339369?dopt=Abstract

Source: Systems Biology in Reproductive Medicine - July 23, 2019 Category: Reproduction Medicine Authors: Paul RK, Balaganur K, Kumar D, Singh R Tags: Syst Biol Reprod Med Source Type: research