Human Th17 cells produce a soluble mediator that increases podocyte motility via signalling pathways which mimic PAR-1 activation.

Human Th17 cells produce a soluble mediator that increases podocyte motility via signalling pathways which mimic PAR-1 activation. Am J Physiol Renal Physiol. 2019 Jul 24;: Authors: May CJ, Welsh GI, Chesor M, Lait PJ, Schewitz-Bowers LP, Lee RW, Saleem MA Abstract The specific pathogenesis of idiopathic nephrotic syndrome (NS) is poorly understood and the role of immune mediators remains contentious. However, there is good evidence for the role of a circulating factor, and we recently postulated circulating proteases as candidate factors. Immunosuppressive therapy with glucocorticoids (GCs) and T cell inhibitors are widely used in the clinical treatment of NS. we hypothesised that Th17 cells produce a circulating factor that is capable of signalling to the podocyte and inducing deleterious phenotypic changes. To test this, we generated human Th17 cells from healthy volunteers and added the supernatants from these T-cell cultures to conditionally immortalised human podocytes in vitro. This demonstrated that podocytes treated with Th17 cell culture supernatant, as well as with patient disease plasma, show significant stimulation of JNK and p38 MAPK pathways and an increase in motility which was blocked using a JNK inhibitor. We have previously shown that nephrotic plasma elicits a podocyte response via the protease receptor PAR-1. Stimulation of PAR-1 in podocytes elicited the same signalling response as Th17 cell culture supernatant treatment. Equally, p...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research

Related Links:

Publication date: September 2019Source: Advances in Chronic Kidney Disease, Volume 26, Issue 5Author(s): Salem Almaani, Samir V. ParikhMembranous lupus nephritis (MLN) (Class V lupus nephritis [LN]) is a distinct form of LN defined by the presence of subepithelial immune complex deposits seen on kidney biopsy. MLN is often associated with the nephrotic syndrome. The histology of MLN closely resembles that of idiopathic (primary) membranous nephropathy (pMN). However, MLN typically has abundant mesangial deposits that are absent in primary membranous nephropathy. The clinical presentation, management, and prognosis of MLN d...
Source: Advances in Chronic Kidney Disease - Category: Urology & Nephrology Source Type: research
Publication date: September 2019Source: Advances in Chronic Kidney Disease, Volume 26, Issue 5Author(s): Nestor Oliva-Damaso, Juan Payan, Elena Oliva-Damaso, Teresa Pereda, Andrew S. BombackIn systemic lupus erythematosus, nephrotic-range proteinuria typically signals the presence of a proliferative lupus nephritis (class III/IV) and/or membranous lupus nephritis (class V, with or without concomitant class III or IV lesions). However, in rare instances, systemic lupus erythematosus patients with nephrotic syndrome have kidney biopsy findings of normal glomeruli or focal segmental glomerulosclerosis lesions, with or without...
Source: Advances in Chronic Kidney Disease - Category: Urology & Nephrology Source Type: research
This study included 227 patients with type 2 diabetes who were admitted to the Department of Nephrology of the Chinese People’s Liberation Army General Hospital from May 2016 to January 2018 and underwent pathological diagnosis by renal biopsy. Anti-PLA2R antibodies were detected by enzyme-linked immunosorbent assay in this population. According to the pathological results, the participants were divided into an IMN group and non-membranous nephropathy (non-MN) group. The clinical characteristics were analyzed, the diagnostic ability of anti-PLA2R antibodies was evaluated, and the receiver operating characteristic (RO...
Source: Clinica Chimica Acta - Category: Laboratory Medicine Source Type: research
AbstractRelapses of steroid-sensitive nephrotic syndrome are traditionally treated with prednisone 2  mg/kg/day or 60 mg/m2/day. Retrospective data support the use of lower doses. We designed a prospective randomized pilot study to investigate the efficacy of different doses in achieving remission of steroid sensitive nephrotic syndrome relapse. The cohort included 30 children with relapsed steroid sensitive nephrotic syndrome, mean age 6.3  ± 3 years and mean disease duration 2.2 ± 1.8 years. The children were randomized to receive 2, 1.5, or 1 mg/kg/day p...
Source: European Journal of Pediatrics - Category: Pediatrics Source Type: research
Praveen Kumar Etta, Sujeeth Reddy, Mulpuru Venkateswar Rao, Madhavi ThatipamulaIndian Journal of Nephrology 2019 29(6):435-437
Source: Indian Journal of Nephrology - Category: Urology & Nephrology Authors: Source Type: research
Conclusions: Tacrolimus is effective in treating refractory multi-drug resistant NS with favorable outcomes in childhood onset NS. PMID: 31705749 [PubMed - in process]
Source: Iranian Journal of Kidney Diseases - Category: Urology & Nephrology Tags: Iran J Kidney Dis Source Type: research
A 78-year-old man with type 2 diabetes, hypertension, chronic anemia and recent-onset nephrotic syndrome presented with a 1-day complain of abdominal pain on his left side. He did not report any other symptoms. In his medical history, he had a partial gastrectomy for cancer 3 years ago
Source: The American Journal of Medicine - Category: General Medicine Authors: Tags: Clinical Communication to the Editor Source Type: research
IgG (mainly IgG3) is the most commonly involved isotype in proliferative glomerulonephritis with monoclonal immunoglobulin deposits (PGNMID). Here we describe the first series of PGNMID with deposition of monoclonal immunoglobulin light chain only (PGNMID-light chain). This multicenter cohort of 17 patients presented with nephritic or nephrotic syndrome with underlying hematologic conditions of monoclonal gammopathy of renal significance (71%) or multiple myeloma (29%). Monoclonal immunoglobulin was identified by serum and urine immunofixation in 65% and 73%, respectively, with abnormal serum free light chain in 83%, and a...
Source: Kidney International - Category: Urology & Nephrology Authors: Tags: Clinical Investigation Source Type: research
AbstractBackgroundRituximab (RTX) is efficient in steroid-dependent nephrotic syndrome (SDNS) in pediatric and adult patients. The aim of this study is to describe hypogammaglobulinemia as a side effect of RTX treatment.MethodsAll pediatric patients (
Source: Pediatric Nephrology - Category: Urology & Nephrology Source Type: research
AbstractA 30-year-old woman on steroid therapy for eosinophilia presented with nephrotic syndrome during steroid tapering. She was diagnosed with membranous nephropathy (MN) stage II –III (positive for IgG1 and IgG4) by renal biopsy. There was no evidence of secondary MN. Her urinary protein level was controlled to 0.5 g/day or less, and her eosinophil count in white blood cell differential was stabilized at less than 10% without increasing the steroid dosage. The renal speci men did not show any enhanced granular expression of PLA2R along the glomerular basement membrane, and PLA2R was not detected in the patie...
Source: CEN Case Reports - Category: Urology & Nephrology Source Type: research
More News: Nephrotic Syndrome | Physiology | Urology & Nephrology | Wales Health