Investigation of intestinal elimination and biliary excretion of ibuprofen in control and hyperglycemic rats.

Investigation of intestinal elimination and biliary excretion of ibuprofen in control and hyperglycemic rats. Can J Physiol Pharmacol. 2019 Jul 24;: Authors: Kovacs NP, Almási A, Garai K, Kuzma M, Vancea S, Fischer E, Perjesi P Abstract An in vivo intestinal perfusion model was used to investigate how experimental hyperglycemia affects intestinal elimination and biliary excretion in the rat. Experimental diabetes was induced by administration of streptozotocin (65mg/kg i.v.). The intestinal perfusion medium contained 250 µM (±)-ibuprofen. An isocratic HPLC method with UV-Vis detection was developed to quantitate ibuprofen in the intestinal perfusate and a gradient method was applied to quantitate ibuprofen and ibuprofen-β-D-glucuronide in the bile. The limit of quantitation of ibuprofen was found to be 0.51 µM in the small intestinal perfusate. In the bile, the limit of quantitation of ibuprofen and ibuprofen-β-D-glucuronide was 4.42 µM and 10.3 µM, respectively. Unconjugated ibuprofen and ibuprofen-β-D-glucuronide were detected in the bile, however, no β-D-glucuronide of ibuprofen could be detected in the intestinal perfusate. The results indicate that experimental diabetes can cause a decrease in the disappearance of ibuprofen from the small intestine. Excretion of both ibuprofen and ibuprofen-β-D-glucuronide decreased to the bile in experimental diabetes. The results can be explained by the results of molecular biologic...
Source: Canadian Journal of Physiology and Pharmacology - Category: Drugs & Pharmacology Authors: Tags: Can J Physiol Pharmacol Source Type: research