Chronic ethanol consumption induces micturition dysfunction and alters the oxidative state of the urinary bladder.

Chronic ethanol consumption induces micturition dysfunction and alters the oxidative state of the urinary bladder. Can J Physiol Pharmacol. 2019 Jul 24;: Authors: Do Vale GT, Sousa AH, Gonzaga NA, De Oliveira MG, Justo AFO, Alexandre EC, Tanus-Santos JE, Antunes E, Tirapelli CR Abstract Oxidative stress is pointed out as a major mechanism by which ethanol induces functional and structural changes in distinctive tissues. We evaluated whether ethanol consumption would increase oxidative stress and cause/span>micturition dysfunction. Male C57BL/6J mice were treated with ethanol 20% (vol./vol.) for 10 weeks. Our findings showed that chronic ethanol consumption reduced micturition spots and urinary volume in conscious mice, whereas in anaesthetized animals cystometric analysis revealed reduced basal pressure and increased capacity, threshold pressure and maximum voiding. Treatment with ethanol reduced the contraction induced by carbachol in isolated bladders. Chronic ethanol consumption increased the levels of oxidant molecules and thiobarbituric acid reactive species (TBARS) in the mice bladder. Up-regulation of Nox2 was detected in the bladder of ethanol-treated mice. Increased activity of both superoxide dismutase (SOD) and catalase were detected in the mouse bladder after treatment with ethanol. Conversely, decreased levels of reduced glutathione (GSH) were detected in the bladder of ethanol-treated mice. The present study first de...
Source: Canadian Journal of Physiology and Pharmacology - Category: Drugs & Pharmacology Authors: Tags: Can J Physiol Pharmacol Source Type: research