Transmission of Multidrug Resistant Campylobacter jejuni to Children from Different Sources in Pakistan

Publication date: Available online 23 July 2019Source: Journal of Global Antimicrobial ResistanceAuthor(s): Zobia Noreen, Fariha Siddiqui, Sundus Javed, Brendan W. Wren, Habib BokhariAbstractObjectivesCampylobacter jejuni has been classified as a member of priority pathogens group due to the rapid emergence of multidrug resistant isolates. In the present study we planned to determine the prevalence, antibiotic resistance patterns and source tracking of clinical C. jejuni isolates from pediatric diarrheal patients in Pakistan.MethodsA total of 150 stool samples from children were processed for the presence of Campylobacter jejuni using culture, biochemical tests and by species specific PCR. Antibiotic susceptibility of the isolates was determined by disc diffusion method and metallo-beta-lactamase (MBL) producers were detected using gene specific PCR. Source tracking was done using source predictive PCR.ResultsCampylobacter jejuni was present in 54.6% of the processed samples. More than 80% of the isolated strains were resistant to 7 out of 12 antibiotics tested. High level of susceptibility was observed against imipenem (12.2%) and tigecycline (9.7%). Six isolates (7.3%) were metallo-beta-lactamase producers and were positive for at least one of the five metallo-beta-lactamase genes. Source tracking showed that 57.3% of the isolates belonged to livestock associated cluster (C1 to C6) and 42.8% were assigned to non-livestock /environmental clusters (C7-C9). Isolates belonging ...
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research

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Publication date: Available online 10 October 2019Source: Comparative Immunology, Microbiology and Infectious DiseasesAuthor(s): Bárbara Martín-Maldonado, Laura Montoro-Dasi, Maria Teresa Pérez-Gracia, Jaume Jordá, Santiago Vega, Francisco Marco-Jiménez, Clara MarinAbstractWild birds have repeatedly been found to be involved in the dissemination of enteric bacterial pathogens in the environment. The aim of this study was to determine the occurrence of Salmonella and Campylobacter as well as the antimicrobial resistance in wild Bonelli’s eagles nestlings in Eastern Spain. In addition...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
ménez F, Marin C Abstract Wild birds have repeatedly been found to be involved in the dissemination of enteric bacterial pathogens in the environment. The aim of this study was to determine the occurrence of Salmonella and Campylobacter as well as the antimicrobial resistance in wild Bonelli's eagles nestlings in Eastern Spain. In addition, we compared the efficiency of two sampling methods (fresh faecal samples from nest and cloacal swabs from nestlings) for detection of both bacteria. A total of 28 nests with 45 nestlings were analysed. In the nest, Salmonella occurrence was 61 ± 9.2%...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research
Abstract Campylobacter jejuni and C. coli are leading causes of human foodborne illness with poultry as a major vehicle. Turkeys are frequently colonized with Campylobacter but little is known about Campylobacter survival in turkey feces, even though fecal droppings are major vehicles for Campylobacter within-flock transmission as well as for environmental dissemination. Our objective was to examine survival of Campylobacter, including different strains, in freshly-excreted feces from naturally-colonized commercial turkey flocks and in suspensions of turkey feces in water from the turkey house. Fecal and water sus...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
The objective of this study was to assess the molecular diversity, using flaA sequencing, of 602 C. jejuni isolated from chicken food chain, i.e. chicken feces (n = 151), chicken carcasses (n = 150), chicken meat (n = 150) and from humans (n = 151) and to determine antimicrobial multiresistant profiles of the isolates as well as to analyze the relationship of the isolate genotypes with their antimicrobial resistance profiles and source of isolation. Multidrug resistant patterns were identified in 110 (18.3%) C. jejuni isolates recovered from all sources and most isolates were resistant to ciprofloxacin (CIP), nalidixic aci...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 29 April 2019Source: Food ControlAuthor(s): Xinfeng Han, Xingai Guan, Hang Zeng, Jiakang Li, Xuelin Huang, Yiping Wen, Qin Zhao, Xiaobo Huang, Qigui Yan, Yong Huang, Sanjie Cao, Rui Wu, Xiaoping Ma, Likou ZouAbstractA total of 220 Campylobacter isolates was recovered from 656 samples along a commercial duck slaughter line after a panel of confirmation tests including selective culture methods, biochemical identification and multiplex PCR in southwestern China. The prevalence of Campylobacter spp. was 33.5%, including 16.6% of Campylobacter jejuni, 15.7% of Campylobacter coli and 3.6% of o...
Source: Food Control - Category: Food Science Source Type: research
Conclusion Genomic analyses herein performed allowed us to confirm the recently (Pérez-Cataluña et al., 2018a,b) suggested amendment of A. butzleri as Al. butzlerii, comb. nov. Antimicrobial susceptibility tests defined Ab 55 and Ab 6V strains as resistant to vancomycin, tetracyclin, nalidixic acid (only Ab 55 whereas Ab 6V is intermediate resistant), erythromycin, and β-lactam antibiotics. Moreover, in our strains isolated from shellfish, we identified numerous virulence, antibiotic, and heavy metal resistance determinants, also additional to those previously found in the genome sequenced A. butzleri ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, association rule mining is an effective tool for identifying patterns of multidrug resistance within antimicrobial susceptibility testing data and evaluating the statistical and biological significance of the patterns. Rule quality measures used to sort and differentiate rules should be tested using resampling procedures to minimize the false discovery rate. Rule mining identified consistent multidrug resistance patterns involving beta-lactams, sulfisoxazole, tetracycline, gentamicin, and streptomycin in E. coli isolated from chicken carcasses and meat between 2004 and 2012. The generally low rule overlap su...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion The present results demonstrated that Chilean salmon farms play an important role as reservoirs of sulfonamide- and trimethoprim-resistant bacteria. The prevalence of conjugative plasmids and integrons among sul-carrying bacteria suggests these bacteria, mainly belonging to the Pseudomonas genus may contribute to high spread of bacterial resistance to sulfonamides and other antibacterials in environments associated with Chilean salmon farms. This is the first study reporting the occurrence of transferable sul and dfr genes and integrons among the antimicrobial resistant bacteria associated with Chilean salmonid...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, a BER homolog encoded by cj0595c (named nth) in C. jejuni was analyzed for endonuclease III activity and for its role in maintaining genomic stability. It was found that inactivation of nth resulted in elevated frequencies of spontaneous fluoroquinolone-resistant (FQR) and oxidative stress resistant (OXR) mutants, compared with the wild-type strain in C. jejuni. Sequencing analysis of the FQR and OXR mutants revealed that the elevated mutation rates were associated with C → T or G → A transition in gyrA (FQR mutants) or perR (for OXR mutants). In an in vitro assay, a purified recombinant C. jejuni ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
AbstractThe aim of this study was to better understand the molecular epidemiology ofCampylobacter coli isolated from multiple sources in Belgium, by studying the genotypic diversity and antimicrobial resistance phenotypes and resistance mechanisms of 59C.  coli isolates. Isolates from broiler carcasses and human cases were genotyped using multilocus sequence typing (MLST),porA typing, flagellin gene A restriction fragment length polymorphism (flaA–RFLP) typing, and by PCR binary typing (P‐BIT). Thirty‐two MLST sequence types, 24flaA types, 31porA alleles, and 29 P ‐BIT types were identified among the scree...
Source: Transboundary and Emerging Diseases - Category: Veterinary Research Authors: Tags: ORIGINAL ARTICLE Source Type: research
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