STAT3 polymorphisms and IL - 6 polymorphism are associated with the risk of basal cell carcinoma in patients from northern Poland

AbstractBasal cell carcinoma (BCC) environment consists of stromal and inflammatory cells which produce variety of cytokines, chemokines and growth factors that may affect tumor behavior. One of the cytokines suggested to be involved in the pathogenesis of BCC is IL-6, which is the upstream element of IL-6/JAK/STAT3 pathway. The correlation between polymorphisms of the genes related to this pathway and cancer risk/prognosis have been previously investigated in several neoplasia, but available data concerning BCC are scarce. In the present study, rs1800795 (-174 G/C)IL-6 gene polymorphism and two polymorphisms in theSTAT3 gene, namely rs2293152 (intron 11, C/G) and rs4796793 (-1697, C/G) were assessed in relation to the BCC risk and clinical course. Additionally, IL-6 serum level was assessed in relation toIL-6 genotype and clinical variables. The study included 254 unrelated patients with BCC and of mean age 70.39  ± 11.43 (69.83 ± 12.32 women, 71.03 ± 10.31 men) and 198 healthy, unrelated age- and sex-matched volunteers.IL-6 andSTAT3 polymorphisms were analyzed using polymerase chain reaction with sequence-specific primers (SSP-PCR). Serum concentration of IL-6 was measured using the ELISA test. We have found that the presence of C allele in rs1800795IL-6 gene polymorphism was associated with increased risk of BCC (aOR 1.86; 95% CI 1.22 –2.84;p = 0.004). The presence of CC genotype inSTAT3 rs2293152 polymorphism was associated with increased BCC risk in ...
Source: Archives of Dermatological Research - Category: Dermatology Source Type: research