The role of oxidative stress in ovarian toxicity induced by haloperidol and clozapine —a histological and biochemical study in albino rats

This study aims to further investigate the role of AP-induced oxidative stress in reproductive dysfunction. Thirty adult female albino rats were divided into three groups including a control group (n = 10) receiving distilled water, HAL group (n = 10) receiving haloperidol (HAL) (2 mg/kg/day), and CLZ group (n = 10) receiving clozapine (CLZ) (20 mg/kg/day). After 28 days, the rats were anesthetized, blood was withdrawn from their hearts, and ovaries were removed before they were sacrificed. Serum prolactin concentrations were measured. For each rat, one ovary was used for biochemical studies includ ing mitochondrial complexes I and III activities and oxidative stress markers (lipid peroxidation, super oxide dismutase [SOD], catalase [CAT], and reduced glutathione [GSH]). The other ovary was used for histopathological examination and immunohistochemistry staining for p53 and Ki-67. HAL-treated rats showed significantly (p <  0.001) higher serum prolactin concentrations compared with other groups. HAL significantly inhibited complexes I (p <  0.001) and III activities (p <  0.05), while CLZ inhibited only complex I (p <  0.001). Lipid peroxidation was increased by HAL (p <  0.001) and CLZ (p <  0.01). HAL caused significant (p <  0.001) reductions in SOD, CAT, and GSH. CLZ caused a significant decrease in SOD (p <  0.001) and GSH (p <  0.01) with no effect on CAT. Histopathological studies of CLZ- and...
Source: Cell and Tissue Research - Category: Cytology Source Type: research