Wnt/ β-catenin signaling was activated in supporting cells during exposure of the zebrafish lateral line to cisplatin.

Wnt/β-catenin signaling was activated in supporting cells during exposure of the zebrafish lateral line to cisplatin. Ann Anat. 2019 Jul 19;: Authors: Mi XX, Yan J, Li Y, Shi JP Abstract Zebrafish lateral line neuromasts are composed of central hair cells surrounded by supporting cells. Cisplatin is a common anticancer drug, with hair cell disruption being a frequent side effect of this drug. In our study, we observed complete functional hair cell loss after six hours of cisplatin insult in neuromasts, as demonstrated by anti-parvalbumin 3 immunofluorescence staining or YO-PRO1 vital dye staining. Time course analysis of neuromast hair cell regeneration showed that regenerated hair cells first appeared between 12 and 24hours after damage, and the abundance of these cells increased stepwise with recovery time. After 72hours, 90% of the hair cells were regenerated, and after 84hours, the number of regenerated hair cells was comparable to the number of neuromast hair cells before treatment. The expression pattern of slc17a8 also showed that hair cells were regenerated after cisplatin exposure. Meanwhile, peripheral supporting cells moved toward the center of the neuromasts, as shown by the in situ expression pattern of sox21a. Increased hair cell progenitor formation was also observed, as demonstrated by the in situ expression pattern of atoh1a. Furthermore, we detected increased expression of wnt2, wnt3a, and ctnnb1 in sorted supporti...
Source: Annals of Anatomy - Category: Anatomy Authors: Tags: Ann Anat Source Type: research