Microfluidic protein isolation and sample preparation for high-resolution cryo-EM [Biophysics and Computational Biology]

High-resolution structural information is essential to understand protein function. Protein-structure determination needs a considerable amount of protein, which can be challenging to produce, often involving harsh and lengthy procedures. In contrast, the several thousand to a few million protein particles required for structure determination by cryogenic electron microscopy (cryo-EM) can...

Source: Proceedings of the National Academy of Sciences - July 22, 2019 Category: Science Authors: Claudio Schmidli, Stefan Albiez, Luca Rima, Ricardo Righetto, Inayatulla Mohammed, Paolo Oliva, Lubomir Kovacik, Henning Stahlberg, Thomas Braun Tags: Biological Sciences Source Type: research