The evolution and transmission of multi-drug resistant Escherichia coli and Klebsiella pneumoniae: the complexity of clones and plasmids.

The evolution and transmission of multi-drug resistant Escherichia coli and Klebsiella pneumoniae: the complexity of clones and plasmids. Curr Opin Microbiol. 2019 Jul 17;51:51-56 Authors: Dunn SJ, Connor C, McNally A Abstract The vast majority of Escherichia coli and Klebsiella pneumoniae isolated from human clinical extra-intestinal infections are now multi-drug resistant (MDR). Extended Spectrum Beta Lactamase (ESBL) carriage in clinical isolates of these bacteria is now commonplace, and carriage of carbapenemases is continuing to increase. MDR is primarily concentrated in a small number of globally disseminated clones, which generally differ between ESBL and carbapenemase carrying-clones in E. coli, but seem to converge in K. pneumoniae. In both species MDR is mediated by acquisition and maintenance of MDR plasmids. The plasmids associated with ESBL and carbapenemases also differ, and when both resistances are present in the same strain they are generally on distinct plasmids. Recent research is attempting to provide clues as to why some lineages appear better suited to acquisition and maintenance of these plasmids without a fitness cost. Central to this is the appearance of adaptive mutations in intergenic regions, and selection on genes involved in anaerobic metabolism, hinting at a process whereby these clones can outcompete commensal strains of the same species to initiate long-term intestinal colonization. PMID: 31325664 [PubMed - as suppli...
Source: Current Opinion in Microbiology - Category: Microbiology Authors: Tags: Curr Opin Microbiol Source Type: research

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In this study, our aim was to evaluate ClO2 precursors, sodium chlorite and citric acid, under disparate but applicable conditions. First, we modeled production of ClO2 from the powdered precursors with three different masses, sample ID 1, 2, and 3, at room (18 °C) and refrigeration (4 °C) temperatures. Second, we investigated the effects of a water reservoir on ClO2 production. Third, we assessed collection method issues that may impact reported ClO2 values. Finally, we evaluated the precursor's efficacy against three common foodborne pathogens: Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella ...
Source: Innovative Food Science and Emerging Technologies - Category: Food Science Source Type: research
Publication date: Available online 26 January 2020Source: Biochemical Engineering JournalAuthor(s): Shujun Wang, Chongyun Sun, Qiushi Hu, Shuang Li, Chengbin Wang, Peng Wang, Lei ZhouAbstractAs foodborne pathogen contamination has become a threat to human health worldwide, early detection of pathogen is significant. Traditional electrochemical immunosensors for pathogen detection involve electrode modification or samples adsorption on the electrode which affect the detection sensitivity and electrode reproducibility, thereby limiting their further application in POCT (point of care testing). We established a homogeneous ma...
Source: Biochemical Engineering Journal - Category: Biochemistry Source Type: research
Conditions:   Immunisation;   Shigella Infection Interventions:   Other: Placebo;   Biological: Shigella sonnei strain 53G;   Biological: WRSs2 Sponsor:   National Institute of Allergy and Infectious Diseases (NIAID) Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
By looking at how disease and infection are controlled at the molecular level, Yale researchers are advancing treatment options for E. coli-related illness.
Source: Yale Science and Health News - Category: Universities & Medical Training Source Type: news
A 57-year-old female with a history of hypertension presented to a local hospital describing one day of intermittent central chest pain that radiated to the jaw.  Five days prior to admission, she attended the Minnesota State Fair where she ate a variety of items including chicken quesadillas. The following day she developed abdominal pain with vomiting and diarrhea. On examination, she was afebrile (36.6 C) and mildly hypertensive to 149/84 mmHg. Cardio-re spiratory and abdominal examinations were unremarkable.
Source: The American Journal of Medicine - Category: General Medicine Authors: Tags: Clinical Communication to the Editor Source Type: research
ConclusionWidespread dissemination ofE. coli strains carryingmcr-1 encoding resistance to colistin in the present study is alarming as this is the last resort drug for the treatment of infections caused by MDR gram-negative bacteria resistant to almost all drugs used commonly.
Source: Gut Pathogens - Category: Microbiology Source Type: research
Publication date: 1 April 2020Source: Materials Letters, Volume 264Author(s): Tauseef Munawar, Sadaf Yasmeen, Altaf Hussain, Muhammad Akram, Faisal IqbalAbstractIn this work, a novel antibacterial and photocatalytic material ZnO-Er2O3-Yb2O3 heterostructured nanocomposite was synthesized by facile co-precipitation technique. The antibacterial activity was performed against S. aureus and E. coli bacteria. The ZOI showed the nanocomposite has higher activity antibacterial against E. coli. The direct optical energy bandgap was 2.9 eV, specified it as a proficient photocatalyst under sunlight illumination. The grown nanoco...
Source: Materials Letters - Category: Materials Science Source Type: research
Anti‑restriction protein KlcAHS enhances carbapenem resistance. Mol Med Rep. 2020 Feb;21(2):903-908 Authors: Liang W, Zhao C, Wang Y, Zhu W, Zhang Y, Hu J, Liu X, Yang L, Zhuang W, Shang Y, Huang G, Shi H Abstract The KlcAHS gene was previously identified as coexisting with the blaKPC‑2 gene in the backbone region of a series of blaKPC‑2‑harboring plasmids. The purpose of the present study was to determine the association between the KlcAHS and blaKPC‑2 genes. KlcAHS deletion and complementation experiments were used to evaluate the association between KlcAHS and carbapenem minimal inhibition...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research
In conclusion, class C1 decoy ODNs inhibited pro‑fibrotic gene expression in rat HSCS by downregulating TGF‑β signaling. PMID: 31974596 [PubMed - in process]
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Publication date: Available online 26 January 2020Source: Food ControlAuthor(s): Hanxu Pan, Kai Dong, Lei Rao, Liang Zhao, Xiaomeng Wu, Yongtao Wang, Xiaojun LiaoAbstractEscherichia coli O157:H7, the causative agent of haemorrhagic colitis and haemolytic uremic syndrome in humans, has been implicated in large food-borne outbreaks all over the world. When confronted with harsh environmental stresses, it can enter into a viable but nonculturable (VBNC) state, which poses a great risk to food safety and public health since conventional methods are invalid to detect VBNC cells. Herein, a system for detecting VBNC state Escheri...
Source: Food Control - Category: Food Science Source Type: research
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