Peptide nucleic acid-mediated re-sensitization of colistin resistance Escherichia coli KP81 harboring mcr-1 plasmid

Publication date: Available online 22 July 2019Source: Microbial PathogenesisAuthor(s): Javad Nezhadi, Hanar Narenji, Mohammad Hossein Soroush Barhaghi, Mohammad Ahangarzadeh Rezaee, Reza Ghotaslou, Tahereh Pirzadeh, Asghar Tanomand, Khudaverdi Ganbarov, Milad Bastami, Masoumeh Madhi, Mehdi Yousefi, Hossein Samadi KafilAbstractEscherichia coli is a gram-negative bacterium and it causes a variety of diseases in humans. It causes a wide range of clinical infections in humans; urinary tract infections is the most prevalent infection caused by uropathogenic Escherichia coli. In recent years, the observation of antibiotic-resistant genes such as resistance to colistin, makes the Escherichia coli resistant to antibiotics like colistin (polymyxin E), because of that the use of new therapies like peptide nucleic acid (PNA) has attracted the consideration of scientists. The aim of this study is the assessment of the inhibitory role of PNA against mcr-1 gene and reduction of mcr-1 gene expression and MIC in colistin resistant E. coli by PNA. NCBI database was used to design PNA. Our study was carried out on E. coli KP81 bacteria containing the mcr-1 gene. Microbroth dilution (MIC) method was used to survey phenotypic sensitivity and determine the sensitivity of the bacteria to the colistin antibiotic. E. coli KP81 isolates were further investigated by polymerase chain reaction to assess the presence of mcr-1 genes and target genes were quantified by real-time PCR assay using specific p...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research