Optimized (Pre) Analytical Conditions and Workflow for Droplet Digital PCR Analysis of Cell-Free DNA from Patients with Suspected Lung Carcinoma
For patients with suspected lung carcinoma, the analysis of circulating tumor DNA, obtained by liquid biopsy, has the potential to support cancer diagnosis and guide targeted therapy. To ensure sensitive and reproducible detection of circulating tumor DNA in routine clinical practice, a standardized (pre) analytical workflow is required. Plasma was obtained from patients and healthy volunteers. Using the QIAmp Circulating Nucleic Acid Kit (Qiagen Valencia, CA), six different procedures for the isolation of cell-free DNA (cfDNA) were compared.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Remco de Kock, Birgit Deiman, Raisa Kraaijvanger, Volkher Scharnhorst Tags: Regular article Source Type: research