First three Escherichia coli isolates harboring mcr-1 in Uruguay
ConclusionsST10 is considered a high-risk clone worldwide. This type of mcr-1-harboring clone is a major concern for human and animal health and must be under close surveillance.In this work we detected the presence of mcr-1 for the first time in our country, albeit in an allodemic manner, associated with different antibiotic resistance genes and from diverse clinical contexts.Considering that colistin is often the last therapeutic option available for multidrug-resistant Gram-Negative Bacilli infections, it is necessary to maximize precautions to avoid the dissemination of isolates carrying mcr-1.
In conclusion, 3.1-PP4 is a highly promising lead towards development of a topical treatment for severely infected skin injuries.
ConclusionThis draft genome can be used as a reference sequence for comparative studies using human and non-human E. coli isolates to identify genetic events that have contributed to pathogenicity and adaptation of TEM-52-producing E. coli clones at the human–animal interface as well as to elucidate dynamics of the spread of blaTEM-52 ESBL genes.
Multidrug resistant pathogens represent one of the biggest global healthcare challenges. Molecular diagnostics can guide effective antibiotics therapy but depends on validated, predictive biomarkers. We here present a novel, generically applicable workflow for rapid identification of antimicrobial resistance (AMR) biomarkers from clinical E. coli isolates and quantitatively evaluate the potential to recover causal biomarkers for observed resistance phenotypes. For this, a metagenomic plasmid library from 1,110 clinical E. coli isolates was created and used for high-throughput screening to identify biomarker candidates agai...
ConclusionThis is the first report showing the high prevalence of carbapenemase among ESBL positive isolates in this study area demanding regular surveillance for such resistance mechanisms which will be useful for health personnel to treat infection by multidrug resistant bacteria.
The emergence of transferable colistin resistance (mcr genes) poses a threat to the effective use of polymyxins, which are last resort drugs to treat infections by multidrug- and carbapenem-resistant Gram-negative bacteria. The aims of the study were to investigate the occurrence of colistin resistance, to determine possible resistance mechanisms and then characterize the mcr-positive Escherichia coli. From the national and EU harmonized antimicrobial resistance monitoring programs, a total of 5,878 commensal E. coli from faecal samples of turkeys, chickens, pigs and cattle collected in 2011-2016 were screened by Minimum...
ConclusionE. coli from contaminated meat sources in Ghana possessed multiple ARGs and were genetically diverse. To the best of our knowledge, this is the first work on WGS of E. coli isolated from various meat samples in the study area. The sequence data add to data base for epidemiological studies.
ConclusionHere we describe E. coli isolates recovered from diarrhoeic piglets and healthy fattening pigs harbouring ESBL and/or mcr-1 genes. 3GC resistance was mainly associated with CTX-M, in particular with blaCTX-M-8/25 alleles.
ConclusionTo our knowledge, this study is the first to report anE. coli ST167 strain co-producing NDM-5, CTX-M-14, OXA-10 and MCR-1 isolated from a sputum sample of an individual with pneumonia in Japan, thus elucidating the molecular characteristics and resistance gene diversity of this strain.
Publication date: Available online 23 July 2019Source: Journal of Global Antimicrobial ResistanceAuthor(s): Zobia Noreen, Fariha Siddiqui, Sundus Javed, Brendan W. Wren, Habib BokhariAbstractObjectivesCampylobacter jejuni has been classified as a member of priority pathogens group due to the rapid emergence of multidrug resistant isolates. In the present study we planned to determine the prevalence, antibiotic resistance patterns and source tracking of clinical C. jejuni isolates from pediatric diarrheal patients in Pakistan.MethodsA total of 150 stool samples from children were processed for the presence of Campylobacter ...
ConclusionThis draft genome can be used as a reference sequence for comparative studies using human and non-human E. coli isolates, in order to identify genetic events that have contributed to pathogenicity and adaptation of TEM-52-producing E. coli clones at the human-animal interface, as well as to elucidate dynamics of the spread of blaTEM-52 ESBL genes.