Garlic and Ginger Extracts Modulated Broiler Chicks Innate Immune Responses and Enhanced Multidrug Resistant Escherichia coli O78 Clearance

This study was designed to determine the in-vitro antibacterial and ex-vivo immunomodulatory efficacy of garlic (Allium sativum) and ginger (Zingiber officinale) extracts post dietary supplementation for 900-one-day-old Sasso broiler chicks. The in-vivo protective action of these extracts against avian pathogenic MDR Escherichia coli (E. coli) O78 challenge was evaluated after 21 days of extracts supplementation. Garlic extract exhibited broader antimicrobial spectra against MDR E. coli O78 and S. aureus isolates. Through the 21 days of garlic or ginger dietary supplementation, the chicks’ innate immune response was modulated via various mechanisms including phagocytosis augmentation, bactericidal activity enhancement and nitric oxide production reduction, together with triggering the IL-1β, IL-6 and IFN-γ cytokines expression levels in comparison with the non-supplemented chicks. It is tempting to speculate that protection against pathogenic E. coli O78 challenge was high in chicks supplemented with each individual extract with severe reduction in the bacterial colony forming units in chicks’ vital organs that confirm the extracts immunomodulatory activity and provide a mechanism(s) of their protective actions. Our data suggest promising useful insights to garlic and ginger dietary supplementation in broilers that may be safe for consumers from antibiotic toxic metabolites’ residues and protective against the risk of infection with bacterial path...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research

Related Links:

Avian pathogenic Escherichia coli (APEC) is the principle cause of colibacillosis affecting poultry. The main challenge to the poultry industry is antimicrobial resistance and the emergence of multidrug resistant...
Source: BMC Veterinary Research - Category: Veterinary Research Authors: Tags: Research article Source Type: research
In this study, we sought to establish a new, carefully optimized method to extract and purify non-denatured, native FliC from the reference UPEC strain CFT073 to be suitable for immune assays. To achieve purification of FliC to homogeneity, we used a mutant CFT073 strain containing deletions in four major chaperone-usher fimbriae operons (type 1, F1C and two P fimbrial gene clusters; CFT073Δ4). A sequential flagella extraction method based on mechanical shearing, ultracentrifugation, size exclusion chromatography, protein concentration and endotoxin removal was applied to CFT073Δ4. Protein purity and integrity ...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
This study revealed that a diverse group of plasmids, carrying AR genes, are responsible for the phenotypic resistance seen in Salmonella isolated from United States food animals. It was also determined that many plasmids carry similar ARCs. Introduction Non-typhoidal Salmonella enterica is one of the most common causes of foodborne illnesses globally, with an estimated 1.2 million cases each year in the United States alone (CDC, 2013). Symptoms range from self-limiting gastrointestinal illness to sepsis. These infections can lead to death unless treated with antibiotics (Crump et al., 2015). Unfortunately, antimic...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This report describes the dynamics of FQ-associated mutations in the highly resistant in FQ mutants in S. Enteritidis. In addition, we characterized a deletion in the ramRA integenic region, demonstrating that this frequent mutation in the highly resistant FQ mutants provide resistance or reduce susceptibility to multiple families of antibiotics. Introduction Non-typhoidal Salmonella (NTS) is a major zoonotic pathogen worldwide (Bangtrakulnonth et al., 2004; Scallan et al., 2011). Infections caused by this pathogen have been mainly associated with gastroenteritis, an acute self-limiting intestinal infection. Howeve...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, we determined the VAGs and antibiotic resistance profiles of APEC isolates recovered from colibacillosis affected broilers in Faisalabad region of Pakistan. A total of 84 diseased and dead birds from different local broilers farms were collected and examined for the gross lesions of colibacillosis by conducting postmortem examination. Of these, APEC isolates were recovered from 75 (89.2%) birds. Antibiotic susceptibility tests against 11 antimicrobial agents showed the highest resistance against ampicillin (98.6%) followed by tetracycline (97.3%) and ciprofloxacin (72%). The presence of 11 virulence-associat...
Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research
This study highlights the public health importance of enhanced antimicrobial resistance surveillance and strict antimicrobial stewardship in human and veterinary healthcare. PMID: 30475200 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research
Publication date: September 2018Source: Microbial Pathogenesis, Volume 122Author(s): Abass Tanhaiean, Marjan Azghandi, Jamshid Razmyar, Elyas Mohammadi, Mohammad Hadi SekhavatiAbstractOver the last decades, poultry industry faced to the rapid emergence of multidrug-resistant bacteria as a global concern. Antimicrobial peptide (AMPs) known as potential antibiotic alternative and were considered as a new antimicrobial agent. Current methods of production and purification of AMPs have several limitations such as: costly, time-consuming and killing the producing host cells in recombinant form. In the present study, a chimeric ...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Extraintestinal pathogenic Escherichia coli (ExPEC) are a phylogenetically diverse group of E. coli that have acquired the ability to colonise anatomical sites distinct from the gastrointestinal tract, including the urinary tract, brain and spinal cord, soft tissue sites and bone. ExPEC are classified into three subtypes, including uropathogenic E. coli (UPEC), neonatal meningitis-associated E. coli and avian pathogenic E. coli [1]. ExPEC also cause nosocomial bloodstream infections in hospitals and nursing homes and are often responsible for respiratory infections and bacteraemia in long-term hospitalised patients.
Source: International Journal of Antimicrobial Agents - Category: Drugs & Pharmacology Authors: Tags: Short Communication Source Type: research
Publication date: September 2018 Source:Microbial Pathogenesis, Volume 122 Author(s): Abass Tanhaiean, Marjan Azghandi, Jamshid Razmyar, Elyas Mohammadi, Mohammad Hadi Sekhavati Over the last decades, poultry industry faced to the rapid emergence of multidrug-resistant bacteria as a global concern. Antimicrobial peptide (AMPs) known as potential antibiotic alternative and were considered as a new antimicrobial agent. Current methods of production and purification of AMPs have several limitations such as: costly, time-consuming and killing the producing host cells in recombinant form. In the present study, a chimeric pepti...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
The 16S rRNA methylase gene rmtB, which encodes a gene product conferring high-level resistance to aminoglycosides, was first described in Serratia marcescens in 2004 [1] and is most prevalent 16S rRNA methylase gene among Enterobacteriaceae strains isolated from humans and animals in China [2 –5]. In addition, rmtB is usually located between the Tn2 transposon and insertion elements such as IS26 and ISCR1 [6,7] and it often coexists with other genes such as blaCTX-M, which encodes an extended-spectrum β-lactamase (ESBL), or fosA3, which confers resistance to fosfomycin in multidrug-re sistant Enterobacteriaceae...
Source: International Journal of Antimicrobial Agents - Category: Drugs & Pharmacology Authors: Tags: Short Communication Source Type: research
More News: Allergy & Immunology | Bird Flu | Diets | Garlic | Gastroenteritis | Ginger | Herbs | Infectious Diseases | Microbiology | Multidrug Resistance | Nutrition | Study | Toxicology