Silica nanoparticle surface chemistry: An important trait affecting cellular biocompatibility in two and three dimensional culture systems

Publication date: 1 October 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 182Author(s): Masoud Hasany, Nayere Taebnia, Soheila Yaghmaei, Mohammad-Ali Shahbazi, Mehdi Mehrali, Alireza Dolatshahi-Pirouz, Ayyoob ArpanaeiAbstractGreat advantages bestowed by mesoporous silica nanoparticles (MSNs) including high surface area, tailorable pore diameter and surface chemistry, and large pore volume render them as efficient tools in biomedical applications. Herein, MSNs with different surface chemistries were synthesized and investigated in terms of biocompatibility and their impact on the morphology of bone marrow-derived mesenchymal stem cells both in 2D and 3D culture systems. Bare MSNs (BMSNs) were synthesized by template removing method using tetraethylorthosilicate (TEOS) as a precursor. The as-prepared BMSNs were then used to prepare amine-functionalized (AMSNs), carboxyl-functionalized (CMSNs) and polymeric amine-functionalized (PMSNs) samples, consecutively. These nanoparticles were characterized by scanning electron microscopy, zeta potential measurement, dynamic light scattering, BET (Brunauer, Emmett, Teller) analysis, and FTIR technique. In a 3D culture system, stem cells were encapsulated in alginate hydrogel in which MSNs of different functionalities were incorporated. The results showed good biocompatibility for both BMSNs and AMSNs in 2D and 3D culture systems. For these samples, the viability of about 80% was acquired after 2 weeks of 3D culture. When comp...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research