TRIM67 promotes NF ‑κB pathway and cell apoptosis in GA‑13315‑treated lung cancer cells.

TRIM67 promotes NF‑κB pathway and cell apoptosis in GA‑13315‑treated lung cancer cells. Mol Med Rep. 2019 Jul 19;: Authors: Liu R, Chen Y, Shou T, Hu J, Chen J, Qing C Abstract 13‑Chlorine‑3,15‑dioxy‑gibberellic acid methyl ester (GA‑13315), a gibberellin derivative, possesses strong anti‑tumor activity in vitro and in vivo. The present study aimed to investigate the underlying mechanisms of GA‑13315‑induced apoptosis in human non‑small cell lung cancer cell lines. Lung cancer cells were treated with different doses of GA‑13315 (4, 8, 16 and 32 ng/µl) for 48 h, and a CCK8 assay was performed to measure cell viability. Alteration in gene expression was identified using RNA‑sequencing (RNA‑Seq). Quantitative polymerase chain reaction (qPCR) was used to confirm the differentially expressed genes (DEGs) identified in RNA‑Seq. Gene expression plasmids or small interfering RNA were used to overexpress or silence targeted genes, in order to investigate downstream signals. Chromatin immunoprecipitation was conducted to evaluate the binding of transcription factors to the target genes. A Student's t‑test or one‑way analysis of variance followed by Tukey's honestly significant difference post‑hoc test were performed to evaluate the significance between groups. P<0.05 was considered to indicate a statistically significant difference. GA‑13315 significantly decreased the number of viable cells and...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research