Degradation of complex casein polymer: Production and optimization of a novel serine metalloprotease from Aspergillus niger KIBGE-IB36

Publication date: Available online 19 July 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Hafsa Sattar, Zainab Bibi, Aysha Kamran, Afsheen Aman, Shah Ali Ul QaderAbstractSerine metalloproteases have substantial scientific interest due to their unique physicochemical properties and capability to degrade complex casein polymer. In this contemporary study, the production of protease was enhanced by optimizing various physicochemical parameters and one-variable at a time approach used under submerged fermentation conditions. Among four different previously isolated Aspergillus species, Aspergillus niger KIBGE-IB36 represented high protease titer along with casein hydrolyzing zone on casein agar plate. Maximum protease production was achieved at 30 °C after 5 days of cultivation period at pH-6.0. Glucose (2.5 gL-1) and casein (2.5 gL-1) augmented protease production within the growth medium. Further increment in protease production was attained when different combinations of nitrogen and mineral salts concentrations used. Peptone (20.0 gL-1), yeast extract (0.50 gL-1), CaCl2 (0.10 gL-1), MgSO4 (0.10 gL-1) and K2HPO4 (0.30 gL-1) boosted the protease secretion. The current study is imperative for the large scale production of serine metalloprotease that also possess extraordinary prominence in food processing industries.
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research

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Conclusion: We describe the first case of fungal necrotising scleritis without intraocular inflammation following pars plana vitrectomy (PubMed Search). Infection should be kept in the differential diagnosis of post-operative necrotising scleritis even in the absence of risk factors like hypopyon or diabetes. Early recognition improves final outcome. Medical therapy should be continued even after presumed cure to take care of residual fungi and prevent recurrences. PMID: 31429609 [PubMed - as supplied by publisher]
Source: Ocular Immunology and Inflammation - Category: Allergy & Immunology Tags: Ocul Immunol Inflamm Source Type: research
This study examined the feasibility oil palm empty fruit bunch (OPEFB) as the source of lignocellulose and Aspergillus niger as cellulase-producing fungus respectively. The hydrolysis was performed by growing A. niger on the OPEFB. Prior to hydrolysis, OPEFB was pretreated with NaOH at different percentage and were heated simultaneously at 170 °C to destroy lignin that hindered cellulase from contacting cellulose. A. niger was further grown on pretreated OPEFB and the reducing sugar was determined via dinitrosalicylic acid method with spectrophotometer reading at 540 nm periodically every 24 h for 9 consecutive d...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
In this study, samples of different kinds of silages (maize, grass, alfalfa, sugar beer pulp, immature corn and ryegrass) collected from farms located in four regions of Spain, were analysed to evaluate the occurrence of aflatoxins (AFs) and Fusarium mycotoxins. Their lactic acid bacteria and fungal populations as well as pH and water activity were also studied. Penicillium (4-26%), Geotrichum (2-21%) and Monascus (0.34-3%) were the main fungi identified in all the silages examined. Aspergillus was found in some maize, grass and alfalfa silage samples and Fusarium was only identified in 0.03% of grass silage samples. The i...
Source: Fungal Biology - Category: Biology Source Type: research
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Source: Food Additives and Contaminants Part A - Category: Food Science Authors: Source Type: research
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Source: Journal of the American Academy of Dermatology - Category: Dermatology Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
The clinical effects of Aspergillus fumigatus in the cystic fibrosis (CF) airway, with the exception of allergic bronchopulmonary aspergillosis, is unclear.
Source: Journal of Cystic Fibrosis - Category: Respiratory Medicine Authors: Source Type: research
In this study, we could biosynthesize stable CuNPs extracellularly by an eco-friendly route using A. versicolor. The biosynthesis of nanoparticles was confirmed by UV-visible spectroscopy, Fourier transform infrared (FTIR), transmission electron microscope (TEM) and dynamic light scattering (DLS) techniques. CuNPs have a size range of 23-82 nm with round to polygonal shape. Antifungal study showed that CuNPs have potential antifungal activity against rotting plant pathogens, where 3.2 and 2.8 µg ml-1 of nanoparticle solution totally inhibited the growth of both Fusarium oxysporum and Phytophthora paras...
Source: Bioprocess and Biosystems Engineering - Category: Biomedical Engineering Authors: Tags: Bioprocess Biosyst Eng Source Type: research
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Source: LWT Food Science and Technology - Category: Food Science Source Type: research
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Source: Food Control - Category: Food Science Source Type: research
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