Optimized dual assay for the transgenes selection and screening in CHO cell line development for recombinant protein production.

CONCLUSIONS: Our method: (i) allows to include into workflow clones that demonstrate slow growth during single cell cloning but producing high amounts of a target protein, which otherwise would be lost in standard protocols of cells screening; (ii) can be applied for testing of DNA vectors for transfection and protein production; (iii) can be used for monitoring the heterogeneity of cell population and analysis of stable pools productivity.
PMID: 31321593 [PubMed - as supplied by publisher]

Source: Biotechnology Letters - July 17, 2019 Category: Biotechnology Authors: Beketova EV, Ibneeva LR, Abdulina YA, Dergousova EA, Filatov VL, Kozlovsky SV, Shilov ES, Datskevich PN, Rozov FN Tags: Biotechnol Lett Source Type: research