Application of genome editing in aquatic farm animals: Atlantic salmon.

Application of genome editing in aquatic farm animals: Atlantic salmon. Transgenic Res. 2019 Aug;28(Suppl 2):101-105 Authors: Wargelius A Abstract Gene editing offers opportunities to solve fish farming sustainability issues that presently hampers expansion of the aquaculture industry. In for example Atlantic salmon farming, there are now two major bottlenecks limiting the expansion of the industry. One is the genetic impact of escaped farmed salmon on wild populations, which is considered the most long-term negative effect on the environment. Secondly and the utmost acute problem is the fish parasite salmon lice, which is currently causing high lethality in wild salmonids due to high concentrations of the parasite in the sea owing to sea cage salmon farming. There are also sustainability issues associated with increased use of vegetable-based ingredients as replacements for marine products in fish feed. This transition comes at the expense of the omega-3 content both in fish feed and the fish filet of the farmed fish. Reduced fish welfare represents another obstacle, and robust farmed fish is needed to avoid negative stress associated phenotypes such as cataract, bone and fin deformities, precocious maturity and higher disease susceptibility. Gene editing could solve some of these problems as genetic traits can be altered positively to reach phenotype of interest such as for example disease resistance and increased omega-3 production. PMID: 3132169...
Source: Transgenic Research - Category: Genetics & Stem Cells Authors: Tags: Transgenic Res Source Type: research

Related Links:

In this study, we cloned and characterized an atg12 homolog from orange spotted grouper (Epinephelus coioides) (Ecatg12). Ecatg12 encodes a 117 amino acid protein that shares 94.0% and 76.8% identity with gourami (Anabas_testudineus) and humans (Homo sapiens), respectively. The transcription level of Ecatg12 was lower in cells infected with Singapore grouper iridovirus (SGIV) than in non-infected cells. Fluorescence microscopy revealed that EcAtg12 localized in the cytoplasm and nucleus in grouper spleen cells. Overexpression of EcAtg12 significantly increased the replication of SGIV, as evidenced by increased severity of ...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
Publication date: October 2019Source: Fish &Shellfish Immunology, Volume 93Author(s): Ebru YilmazAbstractThe present study investigated the effects of dietary anthocyanin on the growth performance, haematological, non-specific immune, and spleen gene expression responses of Nile tilapia, Oreochromis niloticus. Five experimental groups of fish with mean weights of 8.24 ± 0.64 g were used in the study; four of these were fed with diets incorporating anthocyanin (20 mg kg -1, 40 mg kg−1, 80 mg kg−1 and 160 mg kg−1), while the fifth was a control group without dietary anthocya...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
Publication date: October 2019Source: Fish &Shellfish Immunology, Volume 93Author(s): Hye-Jin Go, Chan-Hee Kim, Ji Been Park, Tae Young Kim, Tae Kwan Lee, Hye Young Oh, Nam Gyu ParkAbstractFish skin mucus is considered to act as the first line of defense against waterborne pathogens and to be potential source of novel antimicrobial components. Here we report the purification and characterization of a novel hepcidin type 2-like antimicrobial peptide (TpHAMP2) from the skin mucus of the pufferfish Takifugu pardalis. The purified TpHAMP2 comprised of 23 amino acids (AAs) with eight Cys residues that form four intramolecul...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
In this study, we investigated the influences of dietary iron deficiency on intestinal immune function as well as underlying signaling of on-growing grass carp (Ctenopharyngodon idella). Fish were fed with six graded level of dietary iron for sixty days, and a fourteen days’ challenge test under infection of Aeromonas hydrophila thereafter. Results showed that compared with optimal iron level, iron deficiency increased enteritis morbidity, decreased lysozyme (LZ) and acid phosphatase (ACP) activities, complement 3 (C3), C4 and immunoglobulin M (IgM) concentrations and down-regulated mRNA levels of hepcidin, liver exp...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
Publication date: October 2019Source: Fish &Shellfish Immunology, Volume 93Author(s): Xiujuan Zhou, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Wenbin ZhanAbstractInterleukin-2 receptor subunit beta of flounder (Paralichthys olivace, fIL-2Rβ) was annotated on the NCBI, its gene was cloned and characterized functionally in this study. And then the amino acids sequences and tertiary structure of fIL-2Rβ were analyzed, respectively. RT-PCR and ImageJ analyzed showed that fIL-2Rβ mRNA were expressed in the gill, spleen, kidney, intestines, liver, blood, muscle and skin, which showed high signals in spleen and b...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
In conclusion, our data showed that pan-Trk IHC is a reasonable screening test for diagnosing secretory carcinoma of the salivary gland.
Source: Virchows Archiv - Category: Pathology Source Type: research
This week's case was donated by Florida Fan and one of his coworkers who found this beauty in some fresh salmon:Posterior end: Anterior end:Identification?
Source: Creepy Dreadful Wonderful Parasites - Category: Parasitology Source Type: blogs
Publication date: December 2019Source: Current Opinion in Behavioral Sciences, Volume 30Author(s): Innes C Cuthill, Samuel R Matchette, Nicholas E Scott-SamuelWe review how animals conceal themselves in the face of the need to move, and how this is modulated by the dynamic components and rapidly varying illumination of natural backgrounds. We do so in a framework of minimising the viewer’s signal-to-noise ratio. Motion can match that of the observer such that there is no relative motion cue, or mimic that of background objects (e.g. swaying leaves). For group-living animals, matched motion and colouration constitute ...
Source: Current Opinion in Behavioral Sciences - Category: Psychiatry & Psychology Source Type: research
Publication date: Available online 17 August 2019Source: IDCasesAuthor(s): Mohamadreza Salehi, Sahar Shadvar, Mohammad Sadeghian, Mahsa Doomanlou, Alireza Abdollahi, Seyed Ali Dehghan Manshadi, Akram Sardari, Hossein Ali Rahdar, Mohammad Mehdi FeizabadiAbstractAeromonas salmonicida (A. salmonicida) is a facultative Gram-negative bacillus, inhabiting in water. It is a common source of furunculosis and septicemia in fish. Report on the human infection with this organism is rare. A male farmer referred with weakness and intermittent fever. He had cardiac valves’ regurgitation due to fever with rheumatic heart disease. H...
Source: IDCases - Category: Infectious Diseases Source Type: research
Publication date: Available online 25 July 2019Source: Journal of Clinical and Experimental HepatologyAuthor(s): Sophy Korula, Sumita Danda, Praveen G. Paul, Sarah Mathai, Anna SimonBackgroundGlycogen storage disease (GSD) is typified by early morning seizures. Absence of this results in delayed diagnosis, especially the non-GSD 1 group. Data are limited to few patients with unclear outcome.Objectives1. Study the common presentation and types of GSD. 2. Study the clinical and biochemical outcome. 3. Review genetic mutations.MethodsObservational study from May 2016–April 2019 at metabolic clinic at our cente...
Source: Journal of Clinical and Experimental Hepatology - Category: Gastroenterology Source Type: research
More News: Cataracts | Environmental Health | Fish | Genetics | Lice | Omega 3 | Parasitic Diseases | Parasitology | Vegetables