Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus

Publication date: Available online 19 July 2019Source: Research in Veterinary ScienceAuthor(s): Svetlana Nedić, SlobodankaVakanjac, Marko Samardžija, Sunčica BorozanAbstractThe aim of this study was to determine serum lipid values and parameters of oxidative stress in blood and milk of cows with subclinical mastitis (SCM) caused by Staphylococcus aureus and to establish association between these parameters. The study was performed on total of 104 cows assigned into control group of healthy animals (n = 12) and two groups of cows with SCM, either SCM1 group (n = 37) with ˂ 1000 CFU/mL of S. aureus or SCM2 group (n = 55) with ≥1000 CFU/mL of causative agent in milk. Significantly lower serum concentrations of high density lipoprotein-cholesterol (HDLC) in SCM2 group and higher low density lipoprotein-cholesterol (LDL-C) in both SCM groups were recorded. Significantly lower paraoxonase-1 (PON1) activity and higher lipid hydroperoxides (LOOH) concentration in blood and milk were recorded in both groups of SCM cows vs control. In blood serum of cows from SCM2 group significantly higher concentrations of hydrogen peroxide (H2O2) and malondialdehyde (MDA), but significantly lower total antioxidative capacity (TAC) were obtained. In milk serum of SCM cows were detected significantly higher concentrations of H2O2 and MDA, but significantly lower TAC vs control. There was a significant positive correlation between PON1 in serum with HDLC, but negative correlation ...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research