Tn-sequencing of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis mutant libraries reveals non-essential genes of porcine mycoplasmas differing in pathogenicity

AbstractMycoplasma hyopneumoniae andMycoplasma hyorhinis are two phylogenetically related species colonizing the respiratory tract of pigs but differing in pathogenicity, the basis of which is not well resolved. We hypothesize that genes belonging to the species-specific portion of the genome and being non-essential during ideal laboratory growth conditions encode possible virulent determinants and are the driver of interspecies differences. To investigate this, transposon mutant libraries were generated for both species and a transposon sequencing (Tn-seq) method for mycoplasmas was established to identify non-essential genes. Tn-seq datasets combined with bidirectional Blastp analysis revealed that 101 out of a total 678 coding sequences (CDS) are species-specific and non-essential CDS ofM. hyopneumoniae strain F7.2C, while 96 out of a total 751 CDS are species-specific and non-essential CDS in theM. hyorhinis strain JF5820. Among these species-specific and non-essential CDS were genes involved in metabolic pathways. In particular, the myo-inositol and the sialic acid pathways were found to be non-essential and therefore could be considered important to the specific pathogenicity ofM. hyopneumoniae andM. hyorhinis, respectively. Such pathways could enable the use of an alternative energy source providing an advantage in their specific niche and might be interesting targets to knock out in order to generate attenuated live vaccines.
Source: Veterinary Research - Category: Veterinary Research Source Type: research

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Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Bacterial Infections Source Type: research
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Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Host Response and Inflammation Source Type: research
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Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research
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Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
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Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
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Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research
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Source: International Journal of Colorectal Disease - Category: Gastroenterology Source Type: research
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Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
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Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research
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