Mouse ES-Cell Derived Cardiomyocytes Cease to Beat following Exposure to Monochromatic Light: Associated with Increased ROS and Loss of Calcium Transients.

Mouse ES-Cell Derived Cardiomyocytes Cease to Beat following Exposure to Monochromatic Light: Associated with Increased ROS and Loss of Calcium Transients. Am J Physiol Cell Physiol. 2019 Jul 17;: Authors: Singh G, Sridharan D, Khan M, Seshagiri PB Abstract We earlier established mouse embryonic stem (ES)-cell 'GS-2' line expressing EGFP and have been routinely using it to understand the molecular regulation of differentiation into cardiomyocytes. During such studies, we made a serendipitous discovery that functional cardiomyocytes derived from ES-cells stopped beating when exposed to blue light. We observed a gradual cessation of contractility within a few minutes, regardless of wavelength (nm) ranges tested: blue, green and red with green light manifesting the strongest impact. Following shifting of cultures back into the incubator (darkness), cardiac clusters regained beatings within a few hours. The observed light-induced contractility-inhibition effect was intrinsic to cardiomyocytes and not due to interference from other cell types. Also, this was not influenced by any physicochemical parameters or intracellular EGFP expression. Interestingly, the light-induced cardiomyocyte-contractility inhibition was accompanied by increased intracellular ROS which could be abolished in the presence of N-acetyl cysteine (ROS quencher). Besides, the increased intra-cardiomyocytes' ROS levels was incidental to the inhibition of calcium transie...
Source: Am J Physiol Cell Ph... - Category: Cytology Authors: Tags: Am J Physiol Cell Physiol Source Type: research