The Resolution Matrix in Tomographic Multiplexing: Optimization of Inter-Parameter Cross-Talk, Relative Quantitation, and Localization

Conclusion: The ATD and CCTD inversion methods provide significant advantages over DTD for accurately estimating multiple overlapping fluorophores. Significance: Time domain fluorescence tomography, using zero cross-talk estimators, can serve as a powerful tool for quantifying multiple fluorescently labeled biological processes. The Bayesian framework presented here can be applied to general multiparameter inverse problems for the quantitative estimation of multiple overlapping parameters.
Source: IEEE Transactions on Biomedical Engineering - Category: Biomedical Engineering Source Type: research