Variations of Intracellular Ca2+ Mobilization Initiated by Nanosecond and Microsecond Electrical Pulses in HeLa Cells
Conclusion: These results suggest that the 10-μs PEF takes a large amount of extracellular Na+ into the cell through the electropermeabilized plasma membrane, especially at the anodic side, resulting in the suppression of the Ca2+ influx. On the contrary, the 20-ns-long PEF increased Ca2+ concentration in the surrounding region of the nucleus only in the presence of extracellular Ca2+. The PEF exposure with inhibition of the IP3R indicates that increased Ca2+ ions are released from the ER via the activated IP3R. Significance: These mechanisms could induce specific cell responses, such as Ca2+ oscillations, Ca2+ waves, and Ca2+ puffs.
Source: IEEE Transactions on Biomedical Engineering - Category: Biomedical Engineering Source Type: research
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