The role of DJ-1 in human primary alveolar type II cell injury induced by e-cigarette aerosol.

The role of DJ-1 in human primary alveolar type II cell injury induced by e-cigarette aerosol. Am J Physiol Lung Cell Mol Physiol. 2019 Jul 17;: Authors: Bahmed K, Lin CR, Simborio H, Karim L, Aksoy M, Kelsen S, Tomar D, Muniswamy M, Elrod JW, Messier E, Mason RJ, Unterwald EM, Eisenstein TK, Criner GJ, Kosmider B Abstract The alveolus participates in gas exchange, which can be impaired by environmental factors and toxins. There is an increase in using electronic cigarettes (e-cigarettes); however, their effect on human primary alveolar epithelial cells is unknown. Human lungs were obtained from non-smoker organ donors to isolate alveolar type II (ATII) cells. ATII cells produce and secrete pulmonary surfactant, restore the epithelium after damage and their metabolism highly depends on mitochondrial function. Our data indicates that human ATII cell exposure to e-cigarette aerosol increased IL-8 levels and induced DNA damage and apoptosis. We also studied the cytoprotective effect of DJ-1 against ATII cell injury. DJ-1 knockdown in human primary ATII cells sensitized cells to mitochondrial dysfunction as detected by high mitochondrial superoxide production, decreased mitochondrial membrane potential and calcium elevation. DJ-1 KO mice were more susceptible to ATII cell apoptosis and lung injury induced by e-cigarette aerosol compared to wild-type mice. Regulation of the oxidative phosphorylation (OXPHOS) is important for mitochondrial...
Source: Am J Physiol Lung Ce... - Category: Respiratory Medicine Authors: Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research