Analysis of biological networks in the endothelium with biomimetic microsystem platform.

Analysis of biological networks in the endothelium with biomimetic microsystem platform. Am J Physiol Lung Cell Mol Physiol. 2019 Jul 17;: Authors: Kruse K, Klomp J, Sun M, Chen Z, Santana D, Huang F, Kanabar P, Maienschein-Cline M, Komarova YA Abstract Here we describe a novel method for studying the protein "interactome" in primary human cells and applied this method to investigate the effect of post translational protein modifications (PTMs) on the protein's functions. We created a novel "biomimetic microsystem platform" (Bio-MSP) to isolate the protein complexes in primary cells by covalently attaching purified His-tagged proteins to a solid microscale support. Using this Bio-MSP we have analyzed the "interactomes" of unphosphorylated and phosphomimetic End Binding protein 3 (EB3) in endothelial cells. Pathway analysis of these "interactomes" demonstrated the novel role of EB3 phosphorylation at serine 162 in regulating the protein's function. We showed that phosphorylation "switches" the EB3 biological network to modulate cellular processes such as cell proliferation and cell-cell adhesion. This novel technique provides a useful tool to study the role of PTMs or single point mutations on activating distinct signal transduction networks and thereby biological function of protein in health and disease. PMID: 31313617 [PubMed - as supplied by publisher]
Source: Am J Physiol Lung Ce... - Category: Respiratory Medicine Authors: Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research