Structural insights into flagellar stator-rotor interactions

The bacterial flagellar motor is a molecular machine that can rotate the flagellar filament at high speed. The rotation is generated by the stator-rotor interaction, coupled with an ion flux through the torque-generating stator. Here we employed cryo-electron tomography to visualize the intact flagellar motor in the Lyme disease spirochete,Borrelia burgdorferi. By analyzing the motor structures of wild-type and stator-deletion mutants, we not only localized the stator complexin situ, but also revealed the stator-rotor interaction at an unprecedented detail. Importantly, the stator-rotor interaction induces a conformational change in the flagella C-ring. Given our observation that a non-motile mutant, in which proton flux is blocked, cannot generate the similar conformational change, we propose that the proton-driven torque is responsible for the conformational change required for flagellar rotation.
Source: eLife - Category: Biomedical Science Tags: Microbiology and Infectious Disease Structural Biology and Molecular Biophysics Source Type: research

Related Links:

(Natural News) Patients who have Lyme disease might have an alternative to the ineffective antibiotic-based approach. Ozone therapy offers a more natural and effective way of alleviating the effects of the illness. Lyme disease is a bacterial infection caused by the microbe Borrelia burgdorferi. It spreads to humans through the bites of blood-sucking tick species, such as the black-legged tick and...
Source: NaturalNews.com - Category: Consumer Health News Source Type: news
Publication date: Available online 16 August 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Ilaria Pascucci, Marco Di Domenico, Giulia Capobianco Dondona, Annapia Di Gennaro, Andrea Polci, Andrea Capobianco Dondona, Elisa Mancuso, Cesare Cammà, Giovanni Savini, Jacopo G. Cecere, Fernando Spina, Federica MonacoAbstractThe continuous flow of billions of birds between Africa and Europe creates an “ecological bridge” between physically remote areas. Migratory birds fly south from their breeding grounds during late summer/fall and fly back in spring. These movements regulate the spread of internal and ex...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Tick-borne relapsing fever (TBRF), characterized by recurring febrile episodes, is globally distributed and among the most common bacterial infections in some African countries. Despite the public health concern that this disease represents, little is known regarding the virulence determinants required by TBRF Borrelia during infection. Because the chromosomes of TBRF Borrelia show extensive colinearity with those of Lyme disease (LD) Borrelia, the exceptions represent unique genes encoding proteins that are potentially essential to the disparate enzootic cycles of these two groups of spirochetes. One such exception is a g...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Authors: Serra V, Krey V, Daschkin C, Cafiso A, Sassera D, Maxeiner HG, Modeo L, Nicolaus C, Bandi C, Bazzocchi C Abstract Ixodes ricinus is the most common tick species parasitizing humans in Europe, and the main vector of Borrelia burgdorferi sensu lato, the causative agent of Lyme disease in the continent. This tick species also harbors the endosymbiont Midichloria mitochondrii, and there is strong evidence that this bacterium is inoculated into the vertebrate host during the blood meal. A high proportion of tick bites remains unnoticed due to rarity of immediate symptoms, implying the risk of occult tick-borne ...
Source: Pathogens and Global Health - Category: International Medicine & Public Health Tags: Pathog Glob Health Source Type: research
The modified approach for detecting exposure to Borrelia burgdorferi uses two enzyme immunoassays, with no need for Western blot.FDA Approvals
Source: Medscape Emergency Medicine Headlines - Category: Emergency Medicine Tags: Infectious Diseases News Alert Source Type: news
Discussion Dengue is an important arboviral infection that affects about 40% of the world population. It is found mainly in topical and subtropical areas of the world mainly in developing countries but it range is spreading including the United States. A review of common arboviruses can be found here. It is a flaviavirus with 4 distinct serotypes named DENV-1 through DENV-4 and is spread by A. aegypti a day biting mosquito. Infection with one serotype confers immunity to that serotype but not the others. It does offer some protection for cross-infection but this only lasts a few months. Incubation period is 3-14 days with ...
Source: PediatricEducation.org - Category: Pediatrics Authors: Tags: Uncategorized Source Type: news
(Natural News) Lyme disease is one of the many epidemics that has plagued the United States in recent years. Residents of areas infested by ticks are at considerable risk of contracting the illness. Fortunately, homeopathy offers natural and effective ways. First identified in 1883, Lyme disease originated from pathogenic bacteria called Borrelia burgdorferi. Ticks serve...
Source: NaturalNews.com - Category: Consumer Health News Source Type: news
Updated Date: Jul 16, 2019 EST
Source: DailyMed Drug Label Updates - Category: Drugs & Pharmacology Source Type: alerts
The American Lyme Disease Foundation is dedicated to the prevention, diagnosis, and treatment of Lyme disease which, in the United States, has been defined historically as a tick-borne bacterial infection caused by the spirochete, Borrelia burgdorferi sensu stricto. Through its website, the Foundation plays a major role in providing reliable and scientifically accurate information to the public, medical community and various public health agencies about Lyme disease and its impact on human health.
Source: The American Journal of Medicine - Category: General Medicine Authors: Tags: COMMENTARY Source Type: research
Vector-Borne and Zoonotic Diseases, Ahead of Print.
Source: Vector-Borne and Zoonotic Diseases - Category: Infectious Diseases Authors: Source Type: research
More News: Biology | Biomedical Science | Borrelia | Infectious Diseases | Lyme Disease | Microbiology | Molecular Biology