A comparison between LP(GMA) and CLP(GMA) polymer composites as an immobilization matrix for biosensing applications: A model immunosensor for IL 1 α.

A comparison between LP(GMA) and CLP(GMA) polymer composites as an immobilization matrix for biosensing applications: A model immunosensor for IL 1α. Anal Chim Acta. 2019 Oct 24;1077:129-139 Authors: Aydın EB, Sezgintürk MK Abstract A comparative study was made by using two matrices (Linear poly(glycidyl methacrylate) (GMA) polymer (LP(GMA)) and carbon black-polyvinylidene fluoride (PVDF)-LP(GMA) composite CLP(GMA)) as immobilization platforms for anti-IL 1α antibody on the development of impedimetric immunosensor for IL 1α biomarker determination. These materials were spin coated onto the clean ITO electrode surface separately and used for IL 1α immunosensor fabrication. Carbon black is utilized as a conductive material and has been employed over the last few decades for electrochemical biosensors development. The using of CLP(GMA) composite as an interface material shows fast electron transfer, when compared to LP(GMA) modified ITO electrode. This comparative study investigated the efficacy of carbon black for impedimetric biosensing. Anti-IL 1α antibodies were utilized as bioreceptors and bound to epoxy groups of GMA polymer. For electrochemical characterizations, Electrochemical Impedance Spectroscopy, Cyclic Voltammetry and Single Frequency Impedance methods were employed. Furthermore, to follow the antibody attachment on the modified ITO substrates, Fourier Transform Infrared Spectroscopy and Raman spectral techniques we...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research