Co-aggregation of bacterial flora isolated from the human skin surface

Publication date: Available online 17 July 2019Source: Microbial PathogenesisAuthor(s): K. Vinod Kumar, Archana Pal, Pooja Bai, Andeep Kour, Sheeba E, Rajarajan P, Arbiya Kausar, Monami Chatterjee, Guru Prasad, Swati Balayan, Panchatapa Dutta, Kavinda WijesekeraAbstractHuman hands play a prominent role in the intra and interpersonal transmission of microbes that constantly connect one's microbiome to other individuals and the environment. Along with beneficial bacteria, an individual may harbor pathogenic organisms which may get transferred to others. Thus, understanding the transmission mechanism and interaction among microbiota is crucial in preventing infection. In the present study, the ability of skin microbes, isolated from different individuals, to physically interact (coaggregate) intergenically was assessed. The bacterial flora from the hands (palm area) of similar age group students was isolated. The predominant isolates were selected and identified using 16s rRNA gene sequencing. Further, these isolates were subjected to visual coaggregation assay. A total of 27 bacteria were isolated from the human skin (palm area-fingers) of 10 individuals. These isolates belong to seven bacterial genera and 10 different species. Among 123 combinational visual coaggregation positive reactions; 53.66% showed a reaction score of +1, while 32.52%, 11.38% and 2.44% showed a score of +2, +3, and +4 respectively. Among 27 isolates, Staphylococcus haemolyticus had highest coaggregation ...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research