Prevalence of Mycobacterium avium subsp. paratuberculosis infection in dairy herds in Northern Antioquia (Colombia) and associated risk factors using environmental sampling

Publication date: Available online 15 July 2019Source: Preventive Veterinary MedicineAuthor(s): Nathalia M. Correa-Valencia, Nicolás F. Ramírez-Vásquez, Juan C. Arango-Sabogal, Gilles Fecteau, Jorge A. Fernández-SilvaAbstractThis cross-sectional study aimed to determine Mycobacterium avium subsp. paratuberculosis (MAP) herd-level prevalence using a quantitative real-time PCR method (qPCR), performed on environmental samples. Secondly, the study aimed to explore herd-level risk factors associated with the presence of MAP in dairy herds with in-paddock milking facilities of the Northern region of the Province of Antioquia (Colombia). Study herds (n = 292) located in 61 different districts from six municipalities were randomly selected amongst 7,794 dairies registered in the foot-and-mouth disease vaccination records from 2015. The sampling strategy considered a proportional allocation, both at municipality and district level. Participant herds were visited once between June and October 2016 to collect one composite environmental sample and to complete a risk assessment questionnaire. Each composite environmental sample contained material from six different sites of concentration of adult cattle and/or high traffic areas (e.g. areas surrounding waterers and feeders, areas surrounding the current mobile milking-unit places). Identification of MAP was achieved using a duplex qPCR (Bactotype MAP PCR Kit®, Qiagen). A herd was considered ...
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research

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Publication date: Available online 25 August 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Huseyin Bilgin Bilgic, Selin Hacilarlioglu, Serkan Bakirci, Onur Kose, Ahmet Hakan Unlu, Ayca Aksulu, Metin Pekagirbas, Jabbar Ahmed, Christina Deschermeier, Gordon Langley, Tulin KaragencAbstractBabesiosis is a disease complex caused by unicellular Babesia parasites and among them, malignant ovine babesiosis caused by B. ovis has a devastating economical impact on the small ruminant industry. The control of disease is mainly based on chemotherapy and preventing animals from tick infestation and to date no vaccine is available ...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
The objectives of this retrospective study were to identify the prevalence and distribution of M. ovis, and identify associated demographic and management risk factors, and reproductive and production outcomes associated with infection on domestic sheep (Ovis aries) operations in the United States. As part of the United States Department of Agriculture (USDA), Animal Plant Health Inspection Service, Veterinary Services’ National Animal Health Monitoring System (NAHMS) Sheep 2001 and 2011 studies, blood was collected and sera banked from 21,369 ewes in 2001 and 13,128 ewes in 2011. Participating premises were located ...
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
This study examined the effect of NH3 on the immune response to infectious bronchitis virus (IBV) vaccination and protection against homologous serotype challenge in commercial broiler chickens. One-day-old chicks were administered IBV vaccine and exposed to 30-60 ppm of NH3. At 28 DOA, birds were challenged oculonasally with a pathogenic homologous IBV, and protection was measured by viral detection, clinical signs, ciliostasis, and presence of airsacculitis. IBV-specific serum IgG and lacrimal fluid IgA titers, as well as Harderian gland (HG) immune cell phenotypes, were evaluated. Ammonia exposure was associated ...
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research
Publication date: Available online 23 August 2019Source: Microbial PathogenesisAuthor(s): Lulu Chen, Jialong Zhang, Minmin Wang, Shuonan Pan, Chunxiao Mou, Zhenhai ChenAbstractSeneca Valley virus (SVV) has been identified as the causative agent of SVV-associated vesicular disease (SAVD). To investigate the pathogenicity of two newly isolated SVV strains (GD-S5/2018 and GD04/2017) in China, experimental infections of mice and pigs were performed. SVV GD-S5/2018 was lethal to mice infected by intramuscular, intraperitoneal, or oral routes, while SVV GD04/2017 was avirulent without causing any discernible clinical signs in mi...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
AbstractToll-like receptors (TLRs) are pattern recognition receptors (PRRs) that mediate first line of host defence to pathogens. TLR agonists are potent immunostimulatory agents that help to prime a robust adaptive immune response. In the present study, adjuvant potential of Poly I:C and lipopolysaccharide (LPS) were evaluated with live Newcastle disease virus (NDV) vaccine. Cornish chickens were immunized with live Newcastle disease virus (NDV) vaccine (R2B-mesogenic strain) adjuvanted either with Poly I:C (TLR3 agonist) or LPS-TLR4 agonist and both. Humoral Immune response to ND vaccine was evaluated through haemaggluti...
Source: Veterinary Research Communications - Category: Veterinary Research Source Type: research
Publication date: Available online 20 August 2019Source: Journal of Microbiological MethodsAuthor(s): Fatemeh Rezaei, Mahdi Sharif, Shahabeddin Sarvi, Seyed Hossein Hejazi, Sargis Aghayan, Abdol Sattar Pagheh, Samira Dodangeh, Ahmad DaryaniAbstractToxoplasma gondii is a widespread obligatory intracellular parasite infecting humans and most of all other warm-blooded animals. Currently there is no any accepted vaccine for prevention of T. gondii infection. Many studies are focused on using of various excretory secretory antigens (ESA); and among them dense granule antigens (GRAs) being involved in parasite survival, virulenc...
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research
Globally, tuberculosis (TB) has reemerged as a major cause of morbidity and mortality, despite the use of the M. bovis BCG vaccine and intensive attempts to improve upon BCG or develop new vaccines. Two lacunae in our understanding of the M. tuberculosis (M. tb)-host pathogenesis have mitigated the vaccine efforts; the bacterial-host interaction that enables successful establishment of primary infection and the correlates of protection against TB. The vast majority of vaccine efforts are based on the premise that cell-mediated immunity (CMI) is the predominating mode of protection against TB. However, studies in animal mod...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Publication date: Available online 19 August 2019Source: Veterinary MicrobiologyAuthor(s): Jéssica Caroline Gomes Noll, Lok Raj Joshi, Gabriela Mansano do Nascimento, Maureen Hoch Vieira Fernandes, Bishwas Sharma, Eduardo Furtado Flores, Diego Gustavo DielAbstractCaprine alphaherpesvirus 1 (CpHV-1) is a pathogen associated with systemic infection and respiratory disease in kids and subclinical infection or reproductive failure and abortions in adult goats. The enzyme thymidine kinase (TK) is an important viral product involved in nucleotide synthesis necessary for virus replication. Therefore, it is a common target ...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
This study assessed different dust collection methods for MDV and ILTV detection in a vaccinated layer flock (n = 1700) from day-old to 50 weeks of age. Birds were vaccinated against MDV at day-old, and ILTV by drinking water at week 6 and eye drop at week 12. Dust samples were collected weekly by settle plates (13 plates/15 m2) or by scraping surfaces in the poultry shed and tested for ILTV and MDV genomic copies (GC) by PCR. ILTV GC were detected 4 weeks post water vaccination, peaked at weeks 1214 and became mostly undetectable after week 18. MDV was detected in dust on week 1, peaked at weeks 3-6, decline...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Brucellosis is one of the most prevalent bacterial zoonosis of worldwide distribution. The disease is caused by Brucella spp., facultative intracellular pathogens. Brucellosis in animals results in abortion of fetuses, while in humans, it frequently manifests flu-like symptoms and a typical undulant fever, being osteoarthritis a common complication of the chronic infection. The two most common ways to acquire the infection in humans are through the ingestion of contaminated dairy products or by inhalation of contaminated aerosols. Brucella spp. enter the body mainly through the gastrointestinal and respiratory mucosa; howe...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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