The orphan nuclear receptor NUR77 promotes trophoblast invasion at early pregnancy through paracrine placental growth factor

In this study, we showed that NUR77 expression was significantly lower in the villi of the recurrent miscarriage (RM) group compared to that in the healthy controls (HCs) group. We used immunohistochemistry and found that NUR77 was highly expressed in human placental villi during early pregnancy, especially in syncytiotrophoblast (STB), and was expressed at a much lower level in STB from the RM group than in those from HC group. Western blotting data further confirmed that NUR77 was highly expressed in primary human term placental STB and the FSK-induced BeWo cell line. Moreover, antibody array screening and ELISA revealed that NUR77 promoted significant placental growth factor (PGF) expression during trophoblast fusion. Ectopic overexpression and knockdown experiments demonstrated that PGF was a novel downstream target of NUR77, and serum PGF expression correlated positively with trophoblastNUR77 mRNA levels in HCs and RM patients. Importantly, bioinformatics analysis identified two NUR77 binding sites in the PGF promoter region, and chromatin immunoprecipitation (ChIP) coupled with Western blotting analysis further verified that NUR77 bound directly to the PGF promoter region and promoted PGF expression. Furthermore, in a BeWo/HTR-8 co-culture system, FSK-induced BeWo-secreted PGF promoted HTR-8 cell migration and invasion, and an anti-PGF antibody reversed this effect. Collectively, these results indicated that NUR77 may play a key role in regulating trophoblast invasion a...
Source: Journal of Molecular Medicine - Category: Molecular Biology Source Type: research

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Journal Name: Journal of Perinatal Medicine Issue: Ahead of print
Source: Journal of Perinatal Medicine - Category: Perinatology & Neonatology Source Type: research
CONCLUSION: Thrombophilias are inherited or acquired predispositions to vascular thrombosis and have been associated with thrombosis of the arteriovenous fistula. Patients on hemodialysis with recurrent vascular access thrombosis and presence of thrombophilia should be evaluated about the need for anticoagulant therapy with a vitamin K antagonist. PMID: 31419276 [PubMed - as supplied by publisher]
Source: Jornal Brasileiro de Nefrologia - Category: Urology & Nephrology Tags: J Bras Nefrol Source Type: research
Contributors : Lauretta A Lacko ; Lei Tan ; Shuibing ChenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusTo determine the effect of Zika virus infection on pre-implantation embryonic development, we performed single blastocyst RNA-Seq on MOCK and ZIKV infected embryos. ZIKV infection results in an increased risk of spontaneous abortion and poor intrauterine growth although the mechanisms underlying fetal loss remain undetermined. Little is known about the impact of ZIKV infection during the earliest stages of pregnancy, or pre- and peri-implantation, because most current studies of Z...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
AbstractPurposeTo evaluate the clinical outcomes after fresh transfer of blastocysts cultured from vitrified –thawed cleavage embryos (VTCE) compared with conventional frozen–thawed blastocysts transfer (FBT), or with the usual fresh blastocysts transfer (FRBT).MethodsA total of 155 cycles undergoing fresh transfer of VTCE blastocysts, 4904 cycles undergoing FBT, and 1014 cycles undergoing FRBT were retrospectively analyzed from August 2014 to July 2017. Pregnancy, delivery, and neonatal outcomes were compared after propensity score matching.ResultsVTCE blastocysts ’ transfer resulted in a lower risk of e...
Source: Archives of Gynecology and Obstetrics - Category: OBGYN Source Type: research
ConclusionThe allele frequencies for the assessed genotypes in this study are consistent with the data obtained for other countries. We observed significant susceptible effects of MTHFR C677T, and A1298C among participants. According to the relatively high prevalence of these variants, we recommend genetic testing for women with RPL before therapeutic decisions.
Source: Archives of Gynecology and Obstetrics - Category: OBGYN Source Type: research
Conclusion: The results suggest early routine screening for anxiety and depressive symptoms amongst the Chinese pregnant women with a history of recurrent miscarriage. Health-care professionals should give more effort to enhance these women's social support to decrease their anxiety and depressive symptoms. PMID: 31411054 [PubMed - as supplied by publisher]
Source: Journal of Reproductive and Infant Psychology - Category: Psychiatry & Psychology Tags: J Reprod Infant Psychol Source Type: research
ConclusionSCEM followed by SC appears to be an effective treatment option for CSP. The method seems to be safe in short-term follow-up. However, complications were observed in long-term follow-up. Therefore, patients should be informed about the risk of complications interfering with future fertility, such as intrauterine adhesions and/or amenorrhea.
Source: European Journal of Obstetrics and Gynecology and Reproductive Biology - Category: OBGYN Source Type: research
Publication date: Available online 14 August 2019Source: Meta GeneAuthor(s): Yousef Daneshmandpour, Fariba Pashazadeh, Fereshteh Ansari, Hossein Hosseinifard, Mohammad Nouri, Mehdi Yousefi, Ebrahim SakhiniaAbstract:Assisted Reproduction Techniques (ARTs) are commonly used worldwide, and sperm quality has great effect on its successfulness. Different sperm separation techniques have been developed to increase the quality of used sperms in ART. The aim is to determine the effect of sperm separation techniques including Magnetic Activated Cell Sorting (MACS), Density Gradient Centrifugation (DGC) and Swim up (SU) on pregnancy...
Source: Meta Gene - Category: Genetics & Stem Cells Source Type: research
This study is aimed at understanding CD36 expression, platelet alloantibody distribution in different populations in Northern China, and effects of platelet alloantibodies on pregnancy.Study design and methodsWhole blood samples of 612 subjects including hematological patients, pregnant women, and blood donors were collected at a single center, then CD36 expressions were determined, followed by platelet antibody screening and characterization of platelet antibody specificity. A retrospective analysis was performed in 1552 pregnant women admitted to Department of Obstetrics, in order to investigate FNAIT occurrence.ResultsR...
Source: Clinica Chimica Acta - Category: Laboratory Medicine Source Type: research
ConclusionsGreater likelihood of hospitalization in the ART, MAR, and unassisted subfertile groups is largely explained by admissions for conditions associated with subfertility.
Source: Journal of Assisted Reproduction and Genetics - Category: Reproduction Medicine Source Type: research
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