Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection.

Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection. Methods Mol Biol. 2019;1948:59-68 Authors: Kruse N, Mollenhauer B Abstract Several potential marker candidates for Parkinson's disease (PD) in cerebrospinal fluid (CSF) have been identified. These include α-synuclein, a major constituent of the intracellular aggregates Lewy bodies, a neuropathological hallmark of PD, and others. The extracellular presence of α-synuclein in blood, CSF, saliva, and conditioned media motivated the hypothesis that the quantification of CSF α-synuclein is a biomarker for α-synuclein-related disorders. We here describe the development of an electrochemiluminescence-based assay by conversion of an established ELISA for quantification of α-synuclein. The assay not only works with CSF but can also be used to quantify α-synuclein in different biological fluids, i.e., whole blood and blood products, saliva, as well as brain homogenates and cell culture material. PMID: 30771170 [PubMed - indexed for MEDLINE]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research

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Gambling disorder is a pathologic condition, resulting from the interaction of multiple risk factors. Among these, dopamine neurotransmission is known for playing a significant role, as seen, for example, in patients using dopamine replacement therapy prescribed for Parkinson disease, in which gambling is considered to be iatrogenic. Considering this, together with the general mechanism of action of antipsychotic drugs, growing attention has recently been dedicated to Aripiprazole and to its partial dopaminergic agonism (reducing D2-receptor hyperactivation in the mesolimbic pathway and improving D2-receptor stimulation in...
Source: Addictive Disorders and Their Treatment - Category: Addiction Tags: Case Report Source Type: research
Abstract Cerebrospinal fluid (CSF) has been considered the key source for the search of biomarkers, in particular for neurological diseases, such as Alzheimer's and Parkinson's disease, since it reflects the state of the central nervous system (CNS). Finding biomarkers in the earliest stages of neurodegenerative diseases has become imperative, since, at the moment, there are no drugs that can reverse these pathological processes. Untargeted metabolomics analysis by liquid chromatography combined with SWATH-MS relative quantification is an emerging approach to search for potential biomarkers. In this chapter, we de...
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research
CONCLUSION: No association was found between antihypertensive therapy duration and PD incidence. Further epidemiological studies are needed to compare the effects of subclasses of antihypertensives on PD.
. PMID: 31426904 [PubMed - as supplied by publisher]
Source: International Journal of Clinical Pharmacology and Therapeutics - Category: Drugs & Pharmacology Tags: Int J Clin Pharmacol Ther Source Type: research
Publication date: Available online 20 August 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Basis of DiseaseAuthor(s): Dilshan S. Harischandra, Dharmin Rokad, Shivani Ghaisas, Saurabh Verma, Alan Robertson, Huajun Jin, Vellareddy Anantharam, Arthi Kanthasamy, Anumantha G. KanthasamyAbstractHuman-derived neuronal cell lines are progressively being utilized in understanding neurobiology and preclinical translational research as they are biologically more relevant than rodent-derived cells lines. The Lund human mesencephalic (LUHMES) cell line comprises human neuronal cells that can be differentiated to post-mito...
Source: Biochimica et Biophysica Acta (BBA) Molecular Basis of Disease - Category: Molecular Biology Source Type: research
AbstractRespiratory-swallow coordination (RSC) is important for swallowing safety. Atypical RSC is common in Parkinson ’s disease (PD) and is associated with the presence of dysphagia and aspiration. Verbal cueing is known to affect RSC in healthy adults, yet an understanding of its effect on RSC in PD is unknown. Therefore, the aims of this study were to: (1) assess the effects of verbal cueing on respiratory-swa llow patterning, lung volume initiation, and swallow apnea duration in PD; and (2) determine when during tidal breathing verbal cues should be given in order to increase the likelihood of eliciting optimal ...
Source: Dysphagia - Category: Speech-Language Pathology Source Type: research
Conditions:   Parkinson Disease;   Dyskinesia, Drug-Induced Intervention:   Drug: Intravenous Infusion Sponsor:   Oregon Health and Science University Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Parkinson Disease Interventions:   Other: Clinical Pilates;   Other: Classic Physiotherapy Sponsor:   European University of Lefke Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Conditions:   Parkinson Disease;   Dyskinesia, Drug-Induced Intervention:   Drug: Intravenous Infusion Sponsor:   Oregon Health and Science University Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
In conclusion, lower CC was associated with more severe gait and postural control impairment than patients with upper CC and without CC. Categorizing CC based on the bending fulcrum is compulsory to identify patients with the worst performance and to implement specific rehabilitation programs. PMID: 31428306 [PubMed]
Source: Parkinsons Disease - Category: Neurology Tags: Parkinsons Dis Source Type: research
Authors: Lee W, Williams DR, Evans A Abstract Background: While numerous validated questionnaires measuring psychiatric symptoms in Parkinson's disease (PD) are available, a quick multifaceted screening tool is lacking. Objective: To generate the Brief Psychiatric Questionnaire (BPQ) that quickly screens for depression, anxiety, and apathy and to evaluate its content validity against three reference scales. Methods: Forty-seven questions were drafted and measured against the Geriatric Depression Scale (GDS), State Trait Anxiety Inventory (Form Y2) (STAI-Y2), and Apathy Evaluation Scale (AES). Data were redu...
Source: Parkinsons Disease - Category: Neurology Tags: Parkinsons Dis Source Type: research
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