Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein.

Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein. Methods Mol Biol. 2019;1948:113-121 Authors: Eastwood TA, Mulvihill DP Abstract The majority of proteins in eukaryotic cells are subject to amino-terminal (Nt) acetylation. Recombinant protein expressed using prokaryotic expression systems such as E. coli would not normally be Nt-acetylated as these cells lack the appropriate N-α-terminal acetylation complex. Here we describe a simple protocol that allows the recombinant expression and purification of Nt-acetylated alpha-synuclein (aS) from E. coli. PMID: 30771174 [PubMed - indexed for MEDLINE]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research

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ConclusionsLPS reduced cell proliferation and VEGF secretion of PDLSCs, suggesting that periodontal pathogens might reduce the capability of PDLSCs in periodontal regeneration. Yet, LPS treated PDLSCs remained viable and VEGF secretion increased significantly over time. Further research is needed to study the potential use of PDLSCs in periodontal regeneration and the relationship of biofilm LPS accumulations.
Source: The Saudi Dental Journal - Category: Dentistry Source Type: research
Publication date: December 2019Source: Food Packaging and Shelf Life, Volume 22Author(s): Samira Dehghani, Seyed Hadi Peighambardoust, Seyed Jamaleddin Peighambardoust, Seyed Vali Hosseini, Joe M. RegensteinAbstractThis work dealt with antibacterial nanoparticles of silver (Ag), copper oxide (CuO), and zinc oxide (ZnO) and incorporated their combination at a reduced concentration to low-density polyethylene (LDPE) to prepare active packaging films. SEM (scanning electron microscopy) and SEM-energy dispersive X-ray (EDAX) observations showed a relatively homogenous distribution of nanoparticles on the fracture surfaces of t...
Source: Food Packaging and Shelf Life - Category: Food Science Source Type: research
Authors: Li B, He Y, Ma J, Huang P, Du J, Cao L, Wang Y, Xiao Q, Tang H, Chen S Abstract OBJECTIVE: Gut microbiota changes before the onset of Alzheimer's disease (AD) and the alterations could be detected in the stage of mild cognitive impairment (MCI). The findings might offer diagnostic biomarkers before the onset of dementia. BACKGROUND: AD is the most common cause of dementia, and MCI is the predementia state. Recent studies suggest the alterations in the gut microbial communities associated with AD, whereas the microbiota in MCI before the onset of dementia has not been discovered and characterized in hum...
Source: The Journal of Alzheimers Association - Category: Psychiatry Tags: Alzheimers Dement Source Type: research
Publication date: Available online 22 August 2019Source: Microbial PathogenesisAuthor(s): Waad D. Alrohily, Mahmoud E. Habib, Shahenda M. El-Messery, Abdulmalik Alqurshi, Hussein El-Sabagh, El-Sayed E. HabibAbstractSome synthesized antitumor derivatives of thiazole based chalcones including thiazolo[2,3-b]quinazoline and pyrido[4,3-d]thiazolo[3,2-a]pyrimidine analogues were subjected to be tested against standard microbial strains. Compound 18 showed higher activity against both Gram-positive and Gram-negative bacteria with MIC of 1.0, 1.0, 2.0, 2.0 and 4.0 μg/ml against S. aureus, B. subtilis, M. luteus, E. coli and ...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Abstract Halophilic proteins have greater abundance of acidic over basic residues in sequence. In structure, the surface is decorated by negative charges, with lower content of Lysine. Using sequence BLOCKs and 3D model of malate dehydrogenase from halophilic archaea (Halobacterium salinarum; hsMDH) and X-ray structure from mesophilic bacteria (E. coli; ecMDH), we show that not only acidic and basic residues have higher mean relative abundance (MRA) and thus, impart higher polarity to the sequences, but also show their presence in the surface of the structure of hsMDH relative to its mesophilic counterpart. These ...
Source: Bioinformation - Category: Bioinformatics Authors: Tags: Bioinformation Source Type: research
In this study, biogenic lanthanum (La) nanoparticles were synthesized through ecofriendly green chemistry methodology. Mutingia calabura leaf extract was used for the synthesis of La nanoparticles. The synthesized La nanoparticles were characterized using FTIR, SEM and EDAX. Further, the nanoparticles were analysed for antibacterial activity, bacterial toxicity, antioxidant activity, blood compatibility and dye degradation efficiency. Well diffusion technique was used to study the antibacterial activity and antioxidant activity was evaluated using DPPH assay. Photocatalytic dye degradation study was carried out with Coomas...
Source: Materials Today: Proceedings - Category: Materials Science Source Type: research
Abstract Synthetic dyes are toxic and carcinogenic in nature, which also causes environmental pollution. The present study was aimed to decolorize various commercial dyes using purified recombinant bacterial laccases. Laccase gene from Yersinia enterocolitica strain 8081 (yacK), Y. enterocolitica strain 7 (yacK) and Bacillus pumilus DSKK1 was cloned in vector pET28a and overproduced in host Escherichia coli BL21. The high yield of recombinant laccase protein resulted in the formation of inclusion bodies, which were further solubilized, refolded, and purified. The purified recombinant laccases were alkali-tole...
Source: Environmental Pollution - Category: Environmental Health Authors: Tags: Environ Pollut Source Type: research
Publication date: Available online 21 August 2019Source: Journal of Molecular LiquidsAuthor(s): Jitendra Kumar Sahoo, Monidipa Konar, Juhi Rath, Devendra Kumar, Harekrushna SahooAbstractThe current investigation reports the removal of Eriochrome Black T (EBT) from aqueous solution by using magnetic hydroxyapatite (M-HAP), a cost-effective adsorbent compared to the other commercial adsorbents. The M-HAP nanocomposite was characterized by Fourier transform infrared (FTIR), X-ray diffraction (XRD), Field emission scanning electron microscope (FE-SEM), Environmental scanning electron (E-SEM), BET surface area analyser, vibrati...
Source: Journal of Molecular Liquids - Category: Molecular Biology Source Type: research
In this study, we successfully developed new thiol chitosan-wrapped gold nanoshells (TC-AuNSs) as an antibacterial agent for the near-infrared (NIR) laser-triggered photothermal destruction of antibiotic-resistant pathogens, such as Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), owing to their high water solubility, biocompatibility, strong NIR absorption, and outstanding photothermal properties. More interestingly, TC-AuNSs (115 µg/mL) were capable of completely destroying S. aureus, P. aeruginosa, and E.coli within 5 min of NIR ...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
In this study, LNPs were prepared from corncob lignin via anti-solvent precipitation method, and then characterized by FTIR, TGA, AFM, and NMR. The CNF@CLNPs composite film was fabricated by blending CLNPs with CNF. The results showed that the CLNPs had an average diameter of about 118 nm, meanwhile the main structures and properties of corncob lignin were maintained. The CNF@CLNPs composite film exhibited a high ultraviolet resistance, and a good antibacterial activity against both Escherichia coli and Staphylococcus aureus. It is expected that the CNF@CLNPs composite film would have potential applications in outdo...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
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