Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein.

Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein.
Methods Mol Biol. 2019;1948:113-121
Authors: Eastwood TA, Mulvihill DP
Abstract
The majority of proteins in eukaryotic cells are subject to amino-terminal (Nt) acetylation. Recombinant protein expressed using prokaryotic expression systems such as E. coli would not normally be Nt-acetylated as these cells lack the appropriate N-α-terminal acetylation complex. Here we describe a simple protocol that allows the recombinant expression and purification of Nt-acetylated alpha-synuclein (aS) from E. coli.
PMID: 30771174 [PubMed - indexed for MEDLINE]

Source: Mol Biol Cell - July 16, 2019 Category: Molecular Biology Authors: Eastwood TA, Mulvihill DP Tags: Methods Mol Biol Source Type: research